التفاصيل البيبلوغرافية
| العنوان: |
Defining Changes in the Spatial Distribution and Composition of Brain Lipids in the Shiverer and Cuprizone Mouse Models of Myelin Disease |
| المؤلفون: |
Maganti, Rajanikanth J., Hronowski, Xiaoping L., Dunstan, Robert W., Wipke, Brian T., Zhang, Xueli, Jandreski, Luke, Hamann, Stefan, Juhasz, Peter |
| المصدر: |
Journal of Histochemistry & Cytochemistry ; volume 67, issue 3, page 203-219 ; ISSN 0022-1554 1551-5044 |
| بيانات النشر: |
SAGE Publications |
| سنة النشر: |
2018 |
| الوصف: |
Myelin is composed primarily of lipids and diseases affecting myelin are associated with alterations in its lipid composition. However, correlation of the spatial (in situ) distribution of lipids with the disease-associated compositional and morphological changes is not well defined. Herein we applied high resolution matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS), immunohistochemistry (IHC), and liquid chromatography–electrospray ionization–mass spectrometry (LC-ESI-MS) to evaluate brain lipid alterations in the dysmyelinating shiverer (Shi) mouse and cuprizone (Cz) mouse model of reversible demyelination. MALDI-IMS revealed a decrease in the spatial distribution of sulfatide (SHexCer) species, SHexCer (d42:2), and a phosphatidylcholine (PC) species, PC (36:1), in white matter regions like corpus callosum (CC) both in the Shi mouse and Cz mouse model. Changes in these lipid species were restored albeit not entirely upon spontaneous remyelination after demyelination in the Cz mouse model. Lipid distribution changes correlated with the local morphological changes as confirmed by IHC. LC-ESI-MS analyses of CC extracts confirmed the MALDI-IMS derived reductions in SHexCer and PC species. These findings highlight the role of SHexCer and PC in preserving the normal myelin architecture and our experimental approaches provide a morphological basis to define lipid abnormalities relevant to myelin diseases. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1369/0022155418815860 |
| الإتاحة: |
https://doi.org/10.1369/0022155418815860Test |
| حقوق: |
http://journals.sagepub.com/page/policies/text-and-data-mining-licenseTest |
| رقم الانضمام: |
edsbas.59758CA4 |
| قاعدة البيانات: |
BASE |
