Helicobacter pylori attenuates lipopolysaccharide-induced nitric oxide production by murine macrophages
| العنوان: | Helicobacter pylori attenuates lipopolysaccharide-induced nitric oxide production by murine macrophages |
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| المؤلفون: | Yu Hsin Lin, Hwai Jeng Lin, Ming Chei Maa, Chin Hsin Tang, Wan Chi Lee, Dah Yuu Lu, Chih Ho Lai, Che-Hsin Lee, Chun-Jung Lin, Shih-Hua Fang, Chia Hsian Chang, Yuan-Man Hsu |
| المصدر: | Innate Immunity. 18:406-417 |
| بيانات النشر: | SAGE Publications, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Lipopolysaccharides, Lipopolysaccharide, MAP Kinase Signaling System, Immunology, Nitric Oxide Synthase Type II, Inflammation, Nitric Oxide, Microbiology, Helicobacter Infections, Mice, chemistry.chemical_compound, Immune system, medicine, Animals, Macrophage, Molecular Biology, Immune Evasion, Immunosuppression Therapy, Mice, Knockout, Innate immune system, Helicobacter pylori, biology, Macrophages, NF-kappa B, NF-κB, Cell Biology, NFKB1, biology.organism_classification, Infectious Diseases, chemistry, medicine.symptom |
| الوصف: | Intragastric growth of Helicobacter pylori and non- Helicobacter microorganisms is thought to be associated with elevated levels of pro-inflammatory cytokines and the production of NO these effects can lead to chronic inflammation. Microorganisms can activate the expression of iNOS and the production of NO by macrophages through stimulation with bacterial LPS. Helicobacter pylori can evade these vigorous immune responses, but the underlying mechanism remains unknown. In this study, we used a murine model of macrophage infection to demonstrate that H. pylori inhibits LPS-induced expression of iNOS and production of NO by macrophages. Suppression of LPS-induced NO production by macrophages led to elevated survival of H. pylori in a trans-well system. This effect was abrogated in macrophages from iNOS–/– mice. Analysis of iNOS mRNA and protein levels revealed that H. pylori inhibits iNOS expression at both transcriptional and post-transcriptional levels, and that these effects occurred with live bacteria. Furthermore, the effect of H. pylori involved down-regulation of the mitogen-activated protein kinase pathway and the translocation of active NF-κB into the nucleus. Taken together, our results reveal a new mechanism by which H. pylori modulates the innate immune responses of the host and maintains a persistent infection within the stomach. |
| تدمد: | 1753-4267 1753-4259 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3ae4e244c119b1a751279f46ee4fea4Test https://doi.org/10.1177/1753425911413164Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c3ae4e244c119b1a751279f46ee4fea4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17534267 17534259 |
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