Curcumin improves necrotising microscopic colitis and cell pyroptosis by activating SIRT1/NRF2 and inhibiting the TLR4 signalling pathway in newborn rats
| العنوان: | Curcumin improves necrotising microscopic colitis and cell pyroptosis by activating SIRT1/NRF2 and inhibiting the TLR4 signalling pathway in newborn rats |
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| المؤلفون: | Yiyu Yin, Junhua Cao, Shixian Li, Jian Wang, Xiaole Wu, Huaxin Zou, Bei Peng |
| المصدر: | Innate Immunity Innate Immunity, Vol 26 (2020) |
| بيانات النشر: | SAGE Publications, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | lcsh:Immunologic diseases. Allergy, 0301 basic medicine, Curcumin, Colon, NF-E2-Related Factor 2, Immunology, Cell, H&E stain, Inflammation, Pharmacology, Microbiology, cell pyroptosis, Necrosis, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Microscopic colitis, Sirtuin 1, Pyroptosis, medicine, Animals, Humans, TLR4, Molecular Biology, Cells, Cultured, Anti-Inflammatory Agents, Non-Steroidal, Original Articles, Cell Biology, medicine.disease, necrotising microscopic colitis, digestive system diseases, Rats, Colitis, Microscopic, Toll-Like Receptor 4, Blot, Disease Models, Animal, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Animals, Newborn, chemistry, 030220 oncology & carcinogenesis, medicine.symptom, lcsh:RC581-607, SIRT1/NRF2 |
| الوصف: | This study aimed to explore comprehensively the biological function of curcumin, and its underlying mechanism, in protecting from necrotising microscopic colitis in newborn rats. A total of 20 normal healthy rats were selected, and a necrotising enterocolitis (NEC) model was established. After hypoxia and hypothermia stimulation, these rats were treated with different doses of curcumin (control group, NEC model group, NEC+20 mg/kg curcumin and NEC+50 mg/kg curcumin). Inflammation was identified using hematoxylin and eosin staining, and inflammatory factors were detected via ELISA. The mRNA and protein levels of SIRT1, NRF2, TLR4, NLRP3 and caspase-1 were determined by quantitative RT-PCR and Western blotting, respectively. Curcumin improved the inflammatory condition of NEC and inhibited the expression of inflammatory factors in NEC newborn rat intestinal tissue. Furthermore, the SIRT1/NRF2 pathway was inhibited in the intestinal tissue of NEC newborn rats, whereas curcumin treatment induced the activation of the SIRT1/NRF2 pathway and inhibited TLR4 expression in these animals. In addition, curcumin could also inhibit the expression of inflammatory factors and alleviate the LPS/ATP-induced focal death pathway in intestinal epithelial cells through the SIRT1 pathway. Curcumin can improve necrotising microscopic colitis and cell pyroptosis by attenuating NEC-induced inhibition of SIRT1/NRF2 and inhibiting the TLR4 signalling pathway in newborn rats. |
| تدمد: | 1753-4267 1753-4259 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bea14860177ba55af15ac3af30aaa39Test https://doi.org/10.1177/1753425920933656Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4bea14860177ba55af15ac3af30aaa39 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17534267 17534259 |
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