Activation of Neuropeptide Y Receptors Modulates Retinal Ganglion Cell Physiology and Exerts Neuroprotective Actions In Vitro
| العنوان: | Activation of Neuropeptide Y Receptors Modulates Retinal Ganglion Cell Physiology and Exerts Neuroprotective Actions In Vitro |
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| المؤلفون: | David P.D. Woldbye, Filipe Elvas, Tânia Martins, Serge Picaud, Ana Raquel Santiago, Pedro Tralhão, Miguel Castelo-Branco, António F. Ambrósio, João Martins, Bogdan Kolomiets, Cláudia Cavadas, Dan Brudzewsky, Casper René Gøtzsche |
| المساهمون: | Universidade de Coimbra [Coimbra], Association for Innovation and Biomedical Research on Light and Image (AIBILI), Institut de la Vision, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of Copenhagen = Københavns Universitet (UCPH), HAL UPMC, Gestionnaire, University of Copenhagen = Københavns Universitet (KU) |
| المصدر: | ASN NEURO ASN neuro ASN neuro, 2015, 7 (4), ⟨10.1177/1759091415598292⟩ ASN Neuro, Vol 7 (2015) |
| بيانات النشر: | SAGE Publications, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Retinal Ganglion Cells, genetic structures, chemistry.chemical_compound, Neuropeptide Y, Cells, Cultured, Transcription Factor Brn-3A, medicine.diagnostic_test, General Neuroscience, Glutamate receptor, Neuropeptide Y receptor, humanities, Cell biology, calcium imaging, medicine.anatomical_structure, Retinal ganglion cell, neuromodulation, NMDA receptor, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Original Article, Protein Binding, Agonist, medicine.medical_specialty, medicine.drug_class, Biology, Neuroprotection, lcsh:RC321-571, retinal explants, Retinal Diseases, Internal medicine, mental disorders, Sulfur Isotopes, medicine, Electroretinography, In Situ Nick-End Labeling, Animals, Rats, Long-Evans, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], RNA, Messenger, Rats, Wistar, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, Retinal, electrophysiology, eye diseases, Peptide Fragments, Rats, Receptors, Neuropeptide Y, Disease Models, Animal, Endocrinology, chemistry, Animals, Newborn, Gene Expression Regulation, Guanosine 5'-O-(3-Thiotriphosphate), Calcium, Neurology (clinical), sense organs |
| الوصف: | Neuropeptide Y (NPY) is expressed in mammalian retina but the location and potential modulatory effects of NPY receptor activation remain largely unknown. Retinal ganglion cell (RGC) death is a hallmark of several retinal degenerative diseases, particularly glaucoma. Using purified RGCs and ex vivo rat retinal preparations, we have measured RGC intracellular free calcium concentration ([Ca2+]i) and RGC spiking activity, respectively. We found that NPY attenuated the increase in the [Ca2+]i triggered by glutamate mainly via Y1 receptor activation. Moreover, (Leu31, Pro34)−NPY, a Y1/Y5 receptor agonist, increased the initial burst response of OFF-type RGCs, although no effect was observed on RGC spontaneous spiking activity. The Y1 receptor activation was also able to directly modulate RGC responses by attenuating the NMDA-induced increase in RGC spiking activity. These results suggest that Y1 receptor activation, at the level of inner or outer plexiform layers, leads to modulation of RGC receptive field properties. Using in vitro cultures of rat retinal explants exposed to NMDA, we found that NPY pretreatment prevented NMDA-induced cell death. However, in an animal model of retinal ischemia-reperfusion injury, pretreatment with NPY or (Leu31, Pro34)−NPY was not able to prevent apoptosis or rescue RGCs. In conclusion, we found modulatory effects of NPY application that for the first time were detected at the level of RGCs. However, further studies are needed to evaluate whether NPY neuroprotective actions detected in retinal explants can be translated into animal models of retinal degenerative diseases. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1759-0914 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0040b82fab7396f862a9e7a6c5b3dbeaTest http://europepmc.org/articles/PMC4552225Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0040b82fab7396f862a9e7a6c5b3dbea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17590914 |
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