Amino Acid Sequence of a Major Human Amelogenin Protein Employing Edman Degradation and cDNA Sequencing
| العنوان: | Amino Acid Sequence of a Major Human Amelogenin Protein Employing Edman Degradation and cDNA Sequencing |
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| المؤلفون: | J. Catalano-Sherman, D. Deutsch, A. Palmon, Yigal Burstein |
| المصدر: | Journal of Dental Research. 72:1566-1572 |
| بيانات النشر: | SAGE Publications, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | 0301 basic medicine, Molecular Sequence Data, Biology, Mice, 03 medical and health sciences, Complete sequence, 0302 clinical medicine, Dental Enamel Proteins, Species Specificity, stomatognathic system, Complementary DNA, Animals, Chymotrypsin, Humans, Coding region, Amino Acid Sequence, Cyanogen Bromide, General Dentistry, Peptide sequence, Gene, Chromatography, High Pressure Liquid, Phylogeny, Extracellular Matrix Proteins, Autoanalysis, Amelogenin, Base Sequence, Molecular Structure, Edman degradation, Protein primary structure, Tooth Germ, Sequence Analysis, DNA, 030206 dentistry, Molecular biology, Molecular Weight, 030104 developmental biology, Biochemistry, Cattle, Electrophoresis, Polyacrylamide Gel, Sequence Alignment |
| الوصف: | The abundant hydrophobic, proline-glutamine, and histidine-rich (over 90%) amelogenins constitute the major class of proteins in forming extracellular enamel matrix. These are thought to play a major role in the structural organization and mineralization of developing enamel. The present report describes the successful sequencing of the major human amelogenin protein, by use of both Edman degradation and cDNA sequencing. When Edman degradation was used, over 75% of the primary structure of the protein was determined. This sequence was supplemented with cDNA sequencing studies, which revealed the predicted sequence of this protein. Together, they provide the complete sequence of an important human enamel protein. The information complements recent studies on bovine and human amelogenin genes. A comparison between the present results and the protein sequences predicted from the corresponding human amelogenin genomic coding regions and that of cDNA sequences of other species is described. |
| تدمد: | 1544-0591 0022-0345 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71a09aa5dbb12c101964aa88bb18ee8dTest https://doi.org/10.1177/00220345930720120601Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....71a09aa5dbb12c101964aa88bb18ee8d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15440591 00220345 |
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