المؤلفون: Shan-Shan Lei, Yongsheng Fan, Jie Bao, Du Yu, Ting Zhao, Huan-Peng Gu, Xian-Xian Mei, Li Xu, Shan Wu, Guan-Qun Xie, Xiao-Ping Pan

المصدر: International Journal of Immunopathology and Pharmacology

مصطلحات موضوعية: Adult, Male, Th17/Treg, 0301 basic medicine, medicine.medical_treatment, Immunology, Protein Array Analysis, Enzyme-Linked Immunosorbent Assay, Herpesvirus 1, Human, Disease, medicine.disease_cause, T-Lymphocytes, Regulatory, Immunophenotyping, Flow cytometry, Pathogenesis, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Recurrence, cytokine, Humans, Immunology and Allergy, Outpatient clinic, Medicine, Original Research Article, Immune homeostasis, Pharmacology, medicine.diagnostic_test, business.industry, recurrent herpes labialis, Cell Differentiation, Middle Aged, Flow Cytometry, herpes simplex virus, 030104 developmental biology, Herpes simplex virus, Cytokine, Case-Control Studies, Recurrent herpes labialis, Host-Pathogen Interactions, Cytokines, Th17 Cells, Female, 030211 gastroenterology & hepatology, Herpes Labialis, Inflammation Mediators, business

الوصف: Recurrent herpes labialis (RHL) is a common skin disease that is often caused by herpes simplex virus type I (HSV-1), but its immunology and pathogenesis remain unclear. The balance of Th17/Treg cells is crucial for maintaining immune homeostasis. This study aimed to investigate whether the balance of Th17/Treg cells and related cytokines may be a determinant occurrence in patients with RHL. This is a clinical experimental research based on clinical observation and analysis. We collected RHL patients from the outpatient clinic of the Department of Dermatology of Zhejiang Chinese Medical University (Hangzhou, China) in 2017, conducted questionnaire survey and signed informed consent. Peripheral blood was collected from 30 patients with RHL and 30 healthy volunteers. Flow cytometry was used to detect the percentages of Treg cells and Th17 cells. Protein microarrays coated with 20 cytokines related to T-cell subsets were performed. Enzyme-linked immunosorbent assay (ELISA) assay was conducted to further verify the expression levels of the cytokines that were screened by protein microarrays. Percentages of Th17/Treg cells in peripheral blood of RHL patients were significantly increased compared to those in healthy volunteers. The fold changes of GM-CSF, IL-4, TGF-β, IL-12, IL-10, IL-17F, and TNF-α were significantly increased compared with healthy volunteers. In addition, the expression of IL-4, IL-10, and TGF-β in the serum of RHL patients increased significantly. Our results indicated an imbalance of Th17/Treg cells in RHL, and this imbalance is probably an important factor in the occurrence, development, and recovery of RHL.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4742648ea6c9a74ba8f1ea721475d290Test
https://doi.org/10.1177/2058738420933099Test

المؤلفون: Yan Zhou, Yan-Jie Jia, Jinghong Li, Wencui Wan, Tao Peng, Jinyi Li

المصدر: Journal of International Medical Research. 42:728-736

مصطلحات موضوعية: Adult, Male, Pathology, medicine.medical_specialty, Diagnostic methods, Adolescent, Enzyme-Linked Immunosorbent Assay, urologic and male genital diseases, Biochemistry, Delta like 1, Tuberculous meningitis, Diagnosis, Differential, Cerebrospinal fluid, Meningeal Neoplasms, Viral meningitis, Humans, Medicine, Prospective Studies, Neoplasm Metastasis, CSF albumin, business.industry, Calcium-Binding Proteins, Biochemistry (medical), Membrane Proteins, Mycobacterium tuberculosis, Cell Biology, General Medicine, Middle Aged, medicine.disease, Meningitis, Viral, Case-Control Studies, Tuberculosis, Meningeal, Host-Pathogen Interactions, Intercellular Signaling Peptides and Proteins, Female, Bacterial meningitis, business, Biomarkers, Encephalitis

الوصف: Objective To investigate the diagnostic value of Delta-like 1 ligand (DLL1) in cerebrospinal fluid (CSF) and serum, in tuberculous meningitis (TBM). Methods Patients with a definite diagnosis of central nervous system infection (TBM, viral meningitis/encephalitis or bacterial meningitis) were prospectively enrolled alongside patients with intracranial metastatic tumour and patients with no diagnosis (who served as controls). DLL1 content in CSF and serum was measured quantitatively by enzyme-linked immunosorbent assay; analyses were blinded. Results A total of 173 patients were enrolled: 62 with TBM; 38 with viral meningitis/encephalitis; 26 with bacterial meningitis; 17 with intracranial metastatic tumour; 30 with no diagnosis. CSF DLL1 content was highest for TBM; there were no differences in CSF DLL1 between the other groups. Serum DLL1 content was highest for the TBM and intracranial metastatic tumour groups, with significant differences between the TBM group and the viral meningitis/encephalitis, bacterial meningitis and nondiagnosed groups. There were no differences in serum DLL1 between the viral meningitis/encephalitis, bacterial meningitis and nondiagnosed groups, or between the TBM group and the tumour group. Conclusion As a new biomarker, DLL1 may be of great clinical importance in the diagnosis of TBM.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6507c1c95e50699bc92606485043ed1Test
https://doi.org/10.1177/0300060513498669Test

المؤلفون: Wenyi Qin, Edward R. Sauter, Eva P. Browne, Ke Zhang, Kathleen F. Arcaro, Douglas L. Anderton

المصدر: Journal of Human Lactation. 28:543-546

مصطلحات موضوعية: Adult, Oncology, medicine.medical_specialty, Basic fibroblast growth factor, Breast Neoplasms, Enzyme-Linked Immunosorbent Assay, Breast milk, Lipocalin, Risk Assessment, Transforming Growth Factor beta1, Transforming Growth Factor beta2, chemistry.chemical_compound, Breast cancer, Adipokines, Lipocalin-2, Lectins, Proto-Oncogene Proteins, Internal medicine, Lactation, Biomarkers, Tumor, Carcinoma, Humans, Medicine, Chitinase-3-Like Protein 1, Milk, Human, business.industry, Carcinoma, Ductal, Breast, Case-control study, Obstetrics and Gynecology, Cancer, medicine.disease, Lipocalins, Carcinoma, Intraductal, Noninfiltrating, medicine.anatomical_structure, chemistry, Case-Control Studies, Female, Fibroblast Growth Factor 2, business, Acute-Phase Proteins

الوصف: Background: Breast cancer risk increases during pregnancy and remains elevated for a number of years thereafter. Cancer-associated proteins that are secreted into breast milk may provide a means to detect cancer in the lactating breast or to assess future breast cancer risk. Objective: To determine whether proteins linked to breast cancer would be differentially expressed in matched (both breasts from each participant) human milk samples collected from women with unilateral breast cancer. Methods: Five cancer-associated proteins (basic fibroblast growth factor [bFGF], YKL-40, neutrophil gelatinase-associated lipocalin, and transforming growth factor β1 and β2) were analyzed in milk provided by 5 lactating women, 4 of whom were known to have cancer in 1 breast (and the opposite breast clinically disease free) at the time of milk collection and 1 who developed breast cancer 2 years after milk collection. Results: Expression was significantly higher for TGFβ2 ( P = .03) and bFGF ( P =.03) in the breasts with cancer. Conclusion: These proteins may play a role in assessing a woman’s risk of pregnancy-associated breast cancer. Because of variable protein concentration among patients and the limited sample size, the results are considered preliminary.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e1e727d305cb738dc3e89857f23be10Test
https://doi.org/10.1177/0890334412453205Test

المؤلفون: Marcela Fiol, Jorge Correale

المصدر: Multiple Sclerosis Journal. 17:521-531

مصطلحات موضوعية: Adult, Male, Enzyme-Linked Immunospot Assay, Cellular immunity, Pathology, medicine.medical_specialty, Argentina, Arthritis, Enzyme-Linked Immunosorbent Assay, Inflammatory bowel disease, CHI3L1, Multiple Sclerosis, Relapsing-Remitting, Immune system, Adipokines, Lectins, medicine, Humans, Chitinase-3-Like Protein 1, Cells, Cultured, Glycoproteins, Neuromyelitis optica, business.industry, Multiple sclerosis, ELISPOT, Chitinases, Neuromyelitis Optica, Transendothelial and Transepithelial Migration, Endothelial Cells, Multiple Sclerosis, Chronic Progressive, medicine.disease, Coculture Techniques, Up-Regulation, Myelin-Associated Glycoprotein, Hexosaminidases, Neurology, Blood-Brain Barrier, Case-Control Studies, Immunology, Leukocytes, Mononuclear, Cytokines, Female, Myelin-Oligodendrocyte Glycoprotein, Neurology (clinical), Chemokines, business, Biomarkers, Myelin Proteins

الوصف: Background: Recent studies conducted in arthritis, asthma, and inflammatory bowel disease suggest that chitinases are important in inflammatory processes and tissue remodeling. Objective: To investigate the role of chitinases in multiple sclerosis (MS) and neuromyelitis optica (NMO). Methods: Levels of chitotriosidase, acid mammalian chitinase (AMCase), and chitinase 3-like-1 (CHI3L1) were measured using ELISA, in cerebrospinal fluid (CSF) and in serum from 24 patients with relapsing remitting (RR) MS, 24 patients with secondary progressive (SP) MS, 12 patients with NMO, 24 patients with other inflammatory neurological diseases (OIND), and 24 healthy controls (HCs). The number of anti-MOG cytokine-secreting cells was studied using ELISPOT. Eotaxins, MCP-1, RANTES, and IL-8 were assessed using ELISA. Cell transmigration was determined using an in vitro blood–brain barrier (BBB) model, in the presence and absence of chitinases. Results: CSF chitinase levels were significantly increased in patients with RRMS and NMO compared with HCs and patients with SPMS and OIND. In contrast, no significant differences were detected in serum chitinase levels between groups. Chitinase CSF levels showed correlation with anti-MOG IL-13-producing cells, and eotaxin levels. In vitro experiments showed macrophage chitinase secretion was significantly increased by IL-13, but not by IL-5, IL-6, IL-12, or IFN-γ. Moreover, chitinases enhanced IL-8, RANTES, MCP-1, and eotaxin production, increasing migratory capacity in eosinophils, T cells, and macrophages across an in vitro BBB model. Conclusions: Chitinases increased in the CSF from patients with NMO in response to IL-13. These enhanced levels could contribute to central nervous system inflammation by increasing immune cell migration across the BBB.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b65f48966a7d976f6c5218fce224544eTest
https://doi.org/10.1177/1352458510392619Test

المؤلفون: Javier Cabiedes, Antonio R. Cabral, Donato Alarcón-Segovia

المصدر: Lupus. 13:182-187

مصطلحات موضوعية: Adult, Male, β2 glycoprotein 1, Enzyme-Linked Immunosorbent Assay, Sensitivity and Specificity, Statistics, Nonparametric, Epitope, Cohort Studies, chemistry.chemical_compound, Rheumatology, Reference Values, immune system diseases, Antiphospholipid syndrome, medicine, Cardiolipin, Humans, Lupus Erythematosus, Systemic, Native protein, neoplasms, Autoantibodies, Glycoproteins, Probability, Lupus anticoagulant, biology, business.industry, Middle Aged, Antiphospholipid Syndrome, Prognosis, medicine.disease, chemistry, beta 2-Glycoprotein I, IgG binding, Antibodies, Anticardiolipin, Case-Control Studies, Immunology, Antibodies, Antiphospholipid, biology.protein, Female, Antibody, business, Biomarkers

الوصف: We studied antibodies to beta2-glycoprotein 1 (anti-beta2GP1) from 72 patients with systemic lupus erythematosus (SLE) with or without antiphospholipid syndrome (APS) or with or without anticardiolipin antibodies (aCL). Fifteen patients had APS and positive antiphospholipid antibodies [clinical APS(+)/aPL(+)], 12 patients had APS, negative serum IgG and IgM aCL, antiphosphatidylethanolamine, anti-phosphatidylserine and no lupus anticoagulant [clinical APS(+)/ aPL(-)]. A third group included 16 patients without APS but high aCL levels [clinical APS(-)/ aPL(+)]. In a fourth group we studied 29 patients without clinical manifestations of APS or aCL [clinical APS(-)/aPL(-)]. One hundred anticardiolipin and VDRL-negative normal sera were studied as controls. IgG antibodies to cardiolipin proper in a bovine beta2GP-free system, to human beta2GP1 immobilized on cardiolipin or to human beta2GP1 alone were detected in all sera by ELISA using irradiated and nonirradiated plates from two manufacturers. Sera from APS(+)/aPL(+) patients showed IgG binding to CL, CL + beta2GP1 and beta2GP1 in irradiated and nonirradiated plates. APS(+)/ aPL(-) sera had more significant IgG binding to beta2GP1 than normal controls when studied in both irradiated or nonirradiated plates (P = 0.001). This binding was inhibited by solid-phase cardiolipin in a dose-dependent manner. Sera from the APS(-)/aPL(+) subgroup had comparable IgG activity in both the CL and CL + beta2GP1 assays, while no anti-beta2GP1 activity was detected in these sera. Sera from the clinical APS(-)/aPL(-) patients were negative in the three ELISA systems. Antibodies to human beta2GP1 from SLE patients recognize various epitopes. Those from APS(+)/ aPL(+) patients appear to react with an epitope boosted by cardiolipin in addition to another one present in the native protein. In contrast, anti-beta2GP1 from patients with APS(+)/aPL(-) are blocked by cardiolipin, suggesting that their epitope is the phospholipid-binding site.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afb4e178213a1dd1fb9cd3a50a8e5e4dTest
https://doi.org/10.1191/0961203303lu531oaTest