Cadmium Exposure Enhances Bisphenol A-Induced Genotoxicity through 8-Oxoguanine-DNA Glycosylase-1 OGG1 Inhibition in NIH3T3 Fibroblast Cells
| العنوان: | Cadmium Exposure Enhances Bisphenol A-Induced Genotoxicity through 8-Oxoguanine-DNA Glycosylase-1 OGG1 Inhibition in NIH3T3 Fibroblast Cells |
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| المؤلفون: | Mindi He, Chunhai Chen, Yonghui Lu, Min Li, Lingling Yang, Zhou Zhou, Lei Zhang, Zhi-Yu Chen, Chuan Liu, Zhengping Yu |
| المصدر: | Cellular Physiology and Biochemistry, Vol 39, Iss 3, Pp 961-974 (2016) |
| بيانات النشر: | S. Karger AG, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Physiology, Cytotoxicity, medicine.disease_cause, lcsh:Physiology, DNA Glycosylases, Histones, Toxicology, Mice, chemistry.chemical_compound, Bisphenol A, Cadmium Chloride, lcsh:QD415-436, Phosphorylation, OGG1, chemistry.chemical_classification, Cadmium, lcsh:QP1-981, G2 Phase Cell Cycle Checkpoints, Drug Combinations, Comet Assay, Poly(ADP-ribose) Polymerases, hormones, hormone substitutes, and hormone antagonists, endocrine system, Cell Survival, DNA damage, Poly ADP ribose polymerase, chemistry.chemical_element, Air Pollutants, Occupational, Cadmium chloride, lcsh:Biochemistry, 03 medical and health sciences, Phenols, medicine, Animals, Estrogens, Non-Steroidal, Benzhydryl Compounds, Reactive oxygen species, L-Lactate Dehydrogenase, urogenital system, Molecular biology, Comet assay, 030104 developmental biology, Gene Expression Regulation, chemistry, DNA glycosylase, NIH 3T3 Cells, Genotoxicity, Reactive Oxygen Species, DNA Damage |
| الوصف: | Background: Both cadmium (Cd) and bisphenol A (BPA) are commonly encountered in humans' daily activities, but their combined genotoxic effects remain unclear. Methods: In the present study, we exposed a mouse embryonic fibroblast cell line (NIH3T3) to Cd for 24 h, followed by a 24 h BPA exposure to evaluate toxicity. The cytotoxicity was evaluated by viability with CCK-8 assay and lactate dehydrogenase (LDH) release. Reactive oxygen species (ROS) production was measured by 2′,7′-dichlorofluorescein diacetate (DCFH-DA). And DNA damage was measured by 8-hydroxydeoxyguanosine (8-OHdG), phosphorylated H2AX (γH2AX) and the comet assay. The flow cytometry was used to detect cell cycle distribution, and apoptosis was determined by TUNEL assay and western blot against poly-ADP-ribose polymerase (PARP). Results: The results showed that Cd or BPA treatments alone (with the exception of BPA exposure at 50 μM) did not alter cell viability. However, pre-treatment with Cd aggravated the BPA-induced reduction in cell viability; increased BPA-induced LDH release, ROS production, DNA damage and G2 phase arrest; and elevated BPA-induced TUNEL-positive cells and the expression levels of cleaved PARP. Cd exposure concurrently decreased the expression of 8-oxoguanine-DNA glycosylase-1 (OGG1), whereas OGG1 over-expression abolished the enhancement of Cd on BPA-induced genotoxicity and cytotoxicity. Conclusion: These findings indicate that Cd exposure aggravates BPA-induced genotoxicity and cytotoxicity through OGG1 inhibition. |
| تدمد: | 1421-9778 1015-8987 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::544ee9bb44708eb338f3ea755c3033c2Test https://doi.org/10.1159/000447804Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....544ee9bb44708eb338f3ea755c3033c2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14219778 10158987 |
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