دورية أكاديمية
Anti-Psoriasis Effects and Mechanisms of Α-(8-Quinolinoxy) Zinc Phthalocyanine-Mediated Photodynamic Therapy
العنوان: | Anti-Psoriasis Effects and Mechanisms of Α-(8-Quinolinoxy) Zinc Phthalocyanine-Mediated Photodynamic Therapy |
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المؤلفون: | Liu, Han-Qing, Wang, Ying-Ming, Li, Wan-Fang, Li, Chao, Jiang, Zhi-Huan, Bao, Jie, Wei, Jin-Feng, Jin, Hong-Tao, Wang, Ai-Ping |
المصدر: | Cellular Physiology and Biochemistry ; volume 44, issue 1, page 200-214 ; ISSN 1015-8987 1421-9778 |
بيانات النشر: | S. Karger AG |
سنة النشر: | 2017 |
الوصف: | Background/Aims: The aim of this study was to determine the anti-psoriasis effects of α-(8-quinolinoxy) zinc phthalocyanine (ZnPc-F7)-mediated photodynamic therapy (PDT) and to reveal its mechanisms. Methods: HaCaT cells were used to observe the influence of ZnPc-F7-PDT on cell proliferation in vitro. The in vivo anti-psoriasis effects of ZnPc-F7-PDT were evaluated using a mouse vagina model, a propranolol-induced cavy psoriasis model and an imiquimod (IMQ)-induced nude mouse psoriasis model. Flow cytometry was carried out to determine T lymphocyte levels. Western blotting was performed to determine protein expression, and a reverse transcription-polymerase chain reaction test was performed to determine mRNA expression. Results: The results showed that ZnPc-F7-PDT significantly inhibited the proliferation of HaCaT cells in vitro; when the light doses were fixed, changing the irradiation time or output power had little influence on the inhibition rate. ZnPc-F7-PDT significantly inhibited the hyperproliferation of mouse vaginal epithelium induced by diethylstilbestrol and improved propranolol- and IMQ-induced psoriasis-like symptoms. ZnPc-F7-PDT inhibited IMQ-induced splenomegaly and T lymphocyte abnormalities. ZnPc-F7-PDT did not appear to change T lymphocytes in the mouse vagina model. ZnPc-F7-PDT down-regulated the expression of proliferating cell nuclear antigen (PCNA), B-cell lymphoma-2 (Bcl-2), interleukin (IL)-17A mRNA and IL-17F mRNA, and up-regulated the expression of Bax. Conclusion: In conclusion, ZnPc-F7-PDT exhibited therapeutic effects in psoriasis both in vitro and in vivo and is a potential approach in the treatment of psoriasis. Potential mechanisms of these effects included the inhibition of hyperproliferation; regulation of PCNA, Bcl-2, Bax, IL-17A mRNA and IL-17F mRNA expression; and immune regulation. |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
DOI: | 10.1159/000484647 |
الإتاحة: | https://doi.org/10.1159/000484647Test https://www.karger.com/Article/Pdf/484647Test |
حقوق: | https://creativecommons.org/licenses/by-nc-nd/4.0Test/ ; https://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
رقم الانضمام: | edsbas.BB09CBF8 |
قاعدة البيانات: | BASE |
DOI: | 10.1159/000484647 |
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