دورية أكاديمية
Interleukin-4 and Interleukin-13 Enhance Human Bronchial Smooth Muscle Cell Proliferation
| العنوان: | Interleukin-4 and Interleukin-13 Enhance Human Bronchial Smooth Muscle Cell Proliferation |
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| المؤلفون: | Bossé, Ynuk, Thompson, Charles, Audette, Karine, Stankova, Jana, Rola-Pleszczynski, Marek |
| المصدر: | International Archives of Allergy and Immunology ; volume 146, issue 2, page 138-148 ; ISSN 1018-2438 1423-0097 |
| بيانات النشر: | S. Karger AG |
| سنة النشر: | 2008 |
| الوصف: | Background: T H 2 inflammation and bronchial smooth muscle cell (BSMC) hyperplasia are characteristic features of asthma, but whether these phenomena are linked remains unknown. This study aims to define the effect of the T H 2 cytokines IL-4 and IL-13 on human BSMC proliferation when administered alone or in combination with the fibroblast growth factor 2 (FGF2) growth factor. In addition, the effects of the proinflammatory mediators TNFα and IL-1β and the involvement of members of the well-known family of platelet-derived growth factor (PDGF) mitogens were tested. Methods: BSMC proliferation was measured by crystal violet staining and PDGF and PDGF receptor (PDGFR) expression were determined by RT-PCR, immunocytochemistry, ELISA, flow cytometry and dot plot analysis. Results: Neither IL-4 nor IL-13 alone induced BSMC proliferation, despite both being potent inducers of PDGF-CC. However, following a pretreatment with FGF2, which increased PDGFR α chain expression, both IL-4 and IL-13 increased FGF2-induced BSMC proliferation in a time- and concentration-dependent manner. TNFα and IL-1β did not affect basal or FGF2-induced BSMC proliferation, but both proinflammatory mediators enhanced the proliferative synergism between FGF2 and the T H 2 cytokines. Conclusions: IL-4 and IL-13 potently induce FGF2-primed BSMC proliferation via an autocrine loop involving PDGFRα and PDGF-CC, and this proliferative synergism is amplified by proinflammatory cytokines. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1159/000113517 |
| الإتاحة: | https://doi.org/10.1159/000113517Test https://www.karger.com/Article/Pdf/113517Test |
| حقوق: | https://www.karger.com/Services/SiteLicensesTest ; https://www.karger.com/Services/SiteLicensesTest |
| رقم الانضمام: | edsbas.4324A9F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1159/000113517 |
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