دورية أكاديمية
Alternative G1m, G2m and G3m Allotypes of IGHG Genes Correlate with Atopic and Nonatopic Pathways of Immune Regulation in Children with Bronchial Asthma
| العنوان: | Alternative G1m, G2m and G3m Allotypes of IGHG Genes Correlate with Atopic and Nonatopic Pathways of Immune Regulation in Children with Bronchial Asthma |
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| المؤلفون: | Oxelius, Vivi-Anne, Carlsson, Ann-Margreth, Aurivillius, Magnus |
| المصدر: | International Archives of Allergy and Immunology ; volume 115, issue 3, page 215-219 ; ISSN 1018-2438 1423-0097 |
| بيانات النشر: | S. Karger AG |
| سنة النشر: | 1998 |
| الوصف: | Most genetic studies of bronchial asthma deal with IgE responsiveness. The manner by which allergens trigger IgE production and activate mast cells suggests that several genetic loci may be involved. Several reports of candidate genes include chromosome 6 and HLA antigens, chromosome 14q11 and the α chain of the T cell receptor, chromosome 11q32 and the β chain of the high-affinity IgE receptor and chromosome 5 and the gene cluster for IL-4, respectively. In addition, the immunoglobulin heavy chain G (IGHG) genes on chromosome 14q32 have been associated with both atopic and non atopic bronchial asthma in children. In order to further investigate the role of IGHG genes in asthmatic children, the phenotypes of patients with homozygous but alternative IGHG genes were investigated. IGHG gene expression of patients with childhood asthma was determined by serum Gm allotypes with a quantitative competitive indirect ELISA method. The groups consisted of 24 children with the homozygous G3m(b/b)-G1m(f/f )-G2m(n/n) and 16 with the alternative G3m(g/g)-G1m(a/a)-G2m(–n/–n) genes. The two different genotypes were investigated for serum IgE (PRIST), serum IgG subclass levels (radial immunodiffusion), Gm allotype levels (competitive ELISA), IgA and IgM levels (radial immunodiffusion), peripheral blood eosinophils, specific IgE antibodies (skin prick test, SPT, or radioallergosorbent test, RAST), number of peripheral blood CD lymphocyte markers (flow cytometry) and serum IL-4 and IFN-γ levels (ELISA). Comparison of the two genotypes in children with bronchial asthma revealed significantly increased IgE (p < 0.001), increased specific IgE (p < 0.001), as investigated by SPT or RAST (n = 10 allergens tested), increased number of peripheral blood eosinophils (p < 0.01), increased serum IgG1(f/f )(p < 0.001), IgG2(n/n)(p < 0.001) and IgG3(b/b)(p < 0.01) levels, and decreased CD8 given in percent of the total number of peripheral lymphocytes, (p < 0.02) in the G3m(b/b)-G1m(f/f )-G2m(n/n) genotype. The asthmatic ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1159/000023903 |
| الإتاحة: | https://doi.org/10.1159/000023903Test https://www.karger.com/Article/Pdf/23903Test |
| حقوق: | https://www.karger.com/Services/SiteLicensesTest ; https://www.karger.com/Services/SiteLicensesTest |
| رقم الانضمام: | edsbas.9FA2EFD6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1159/000023903 |
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