Lymphocyte Subsets in Cervicovaginal Lavage Specimens of HIV-Infected Women: A Surrogate Risk Marker of HPV-Associated Cervical Lesions
| العنوان: | Lymphocyte Subsets in Cervicovaginal Lavage Specimens of HIV-Infected Women: A Surrogate Risk Marker of HPV-Associated Cervical Lesions |
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| المؤلفون: | Maria Jose Brito, Ana Quintas, Filipa Passos, Ana Teresa Alves, Ana Félix, Catarina Gregório Martins |
| المصدر: | Pathobiology. :1-11 |
| بيانات النشر: | S. Karger AG, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Cell Biology, General Medicine, Molecular Biology, Pathology and Forensic Medicine |
| الوصف: | Introduction: To better understand the role of mucosa immunity in the development of cervical carcinoma in HIV infection, cervical lymphocyte subsets were characterized in HIV+ and HIV− women, as well as their relation to HPV-associated cervical lesions. Methods: Eighty-three (52 HIV+, 31 HIV−) cell suspensions of cervicovaginal lavage (CVL) and 52 HIV+ peripheral blood (PB) samples were assessed by flow cytometry to evaluate lymphoid populations. High-risk (HR) HPV was assessed in liquid-based cytology and HIV mRNA in PB in the same patients. Results: Cervical CD4+ T cells and CD4+/CD8+ ratio were decreased (p < 0.0001) and cervical CD8+ T cells were increased (p = 0.0080) in HIV+ women. These patients had lower CD4+ T-cell percentages in CVL compared to PB (p = 0.0257), and the opposite was true for CD8+ T cells (p = 0.0104). They also had a higher prevalence of high-grade squamous intraepithelial lesions (SILs) with an increased prevalence of HR HPV. Cervical CD8+ T cells were increased in HR HPV+ patients (p = 0.0300) and related to higher prevalence of SILs (p = 0.0001). Discussion/Conclusion: Cervical lymphoid populations can be characterized by flow cytometry, showing a distinct cervical T-cell compartment in HIV+ women. This may represent a surrogate risk marker of HPV-associated cervical lesions in this population and prompt further research on this subject, contributing to improving patients’ management. |
| تدمد: | 1423-0291 1015-2008 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::acc391f2808130a19614bc61b14a3edcTest https://doi.org/10.1159/000526965Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....acc391f2808130a19614bc61b14a3edc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14230291 10152008 |
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