MicroRNA-148a Suppresses Invasion and Metastasis of Human Non-Small-Cell Lung Cancer
| العنوان: | MicroRNA-148a Suppresses Invasion and Metastasis of Human Non-Small-Cell Lung Cancer |
|---|---|
| المؤلفون: | Hanwei Kong, Ming Yao, Jing Li, Lei Liu, Hechun Lin, Dandan Chu, Miaoxin Zhu, Jun Cao, Ying Liu, Tao Yu, Mingxia Yan |
| المصدر: | Cellular Physiology and Biochemistry, Vol 37, Iss 5, Pp 1847-1856 (2015) |
| بيانات النشر: | S. Karger AG, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Oncology, medicine.medical_specialty, Lung Neoplasms, Physiology, Down-Regulation, Mice, Nude, Biology, Real-Time Polymerase Chain Reaction, NSCLC, lcsh:Physiology, Metastasis, lcsh:Biochemistry, Mice, Downregulation and upregulation, Cell Movement, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, Internal medicine, microRNA, medicine, Carcinoma, Animals, Humans, lcsh:QD415-436, Lung cancer, Lung, Aged, Mice, Inbred BALB C, lcsh:QP1-981, Oligonucleotide, Middle Aged, Oligonucleotides, Antisense, medicine.disease, respiratory tract diseases, MicroRNAs, Real-time polymerase chain reaction, MiR-148a, Cell culture, Lymphatic Metastasis, Cancer research, Female |
| الوصف: | Background/Aims: microRNAs (miRNAs) are noncoding RNAs that regulate multiple targets through either the degradation of mRNAs or the inhibition of protein translation, thereby altering several functions simultaneously. Growing evidence indicates that miRNAs are involved in carcinogenesis and tumor progression in non-small-cell lung cancer (NSCLC). Methods: In this study, the mRNA expression levels of miR-148a were examined in NSCLC cell lines and patient specimens using quantitative reverse transcription-PCR. The functions of miR-148a in migration/invasion and lung metastasis formation were determined by using transwell and tail vein injection assays, respectively. Results: We demonstrated that miR-148a was down-regulated in NSCLC metastatic samples, and its expression was suppressed in NSCLC compared with the corresponding nonmalignant lung tissues. Clinical analysis indicated that miR-148a expression was lower in NSCLC patients compared with nonmalignant lung tissues . Decreased miR-148a was significantly associated with tumor node metastasis stage and lymph node metastasis. Furthermore, functional assays showed that miR-148a expression suppressed NSCLC cell invasive and migratory abilities in vitro and suppressed cancer metastasis in vivo, while inhibition of miR-148a enhanced NSCLC cell invasion and lung metastasis formation in a mouse model. Conclusions: Evidence from this study demonstrated that miR-148a exerts tumor-suppressive effects in NSCLC and suggests a new therapeutic option for NSCLC. |
| تدمد: | 1421-9778 1015-8987 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56f9a139e4b20f557f76dbcc453de57aTest https://doi.org/10.1159/000438546Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....56f9a139e4b20f557f76dbcc453de57a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14219778 10158987 |
|---|