Induction of Leucocyte Recruitment and Bronchial Hyperresponsiveness in the Guinea Pig by Aerosol Administration of lnterleukin-2
| العنوان: | Induction of Leucocyte Recruitment and Bronchial Hyperresponsiveness in the Guinea Pig by Aerosol Administration of lnterleukin-2 |
|---|---|
| المؤلفون: | Paul G. Hellewell, Priscilla J. Piper, AileenA.Y. Milne, Mauro M. Teixeira |
| المصدر: | International Archives of Allergy and Immunology. 108:60-67 |
| بيانات النشر: | S. Karger AG, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Male, Interleukin 2, Cellular immunity, Ovalbumin, medicine.medical_treatment, Guinea Pigs, Immunology, Guinea pig, Administration, Inhalation, medicine, Animals, Humans, Immunology and Allergy, Interleukin 5, Aerosols, medicine.diagnostic_test, business.industry, Antibodies, Monoclonal, General Medicine, respiratory system, medicine.disease, Recombinant Proteins, Chemotaxis, Leukocyte, Bronchoalveolar lavage, Cytokine, medicine.anatomical_structure, Bronchial hyperresponsiveness, Interleukin-2, Bronchial Hyperreactivity, Interleukin-5, business, medicine.drug, Respiratory tract |
| الوصف: | Exposure of guinea pigs to an aerosol of human recombinant interleukin-2 (IL-2; 30 micrograms) resulted in an increase in the numbers of eosinophils and macrophages recovered from bronchoalveolar lavage fluid (BALF) 24 h later. This was accompanied by a bronchial hyperresponsiveness to intravenous acetylcholine. In guinea pigs sensitized to ovalbumin, exposure to IL-2 caused an increase in the number of macrophages, but not eosinophils in BALF and bronchial hyperresponsiveness to acetylcholine did not develop. In guinea pig skin, intradermal injection of IL-2 (10(-14) to 10(-9) mol/site) had no effect on 111In-eosinophil accumulation, measured over 3 h, suggesting that IL-2 does not act directly to recruit eosinophils. The hypothesis that IL-2 may be acting via release of interleukin-5 (IL-5) was tested using an antibody to IL-5 (TRFK-5; 1 mg/kg). Treatment with TRFK-5 1 h before exposure to IL-2 aerosol had no effect on the numbers of macrophages or eosinophils recovered from BALF 24 h later, although there was a tendency for reduced bronchial hyperresponsiveness to acetylcholine. These results suggest that (1) IL-2 is not a directly acting chemoattractant for eosinophils in the guinea pig, (2) the action of IL-2 to increase bronchial hyperresponsiveness is also indirect, partly via generation of IL-5, and (3) immunological sensitization alters the response of both eosinophils and bronchial smooth muscle to IL-2. |
| تدمد: | 1423-0097 1018-2438 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c728c7a0197c5e5ceea00fc4dc3dadbTest https://doi.org/10.1159/000237119Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....4c728c7a0197c5e5ceea00fc4dc3dadb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14230097 10182438 |
|---|