The Potential Allergenicity of Two 2S Albumins from Soybean (Glycine max): A Protein Microarray Approach
| العنوان: | The Potential Allergenicity of Two 2S Albumins from Soybean (Glycine max): A Protein Microarray Approach |
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| المؤلفون: | P B Wilson, David B. Archer, Helmut Haas, Kirsten Beyer, Peter R. Shewry, Bodo Niggemann, Marcos J. C. Alcocer, Jing Lin |
| المصدر: | International Archives of Allergy and Immunology. 141:91-102 |
| بيانات النشر: | S. Karger AG, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Immunology, Albumin, food and beverages, General Medicine, Biology, Trypsin, Biochemistry, chemistry, medicine, biology.protein, Protein microarray, Immunology and Allergy, Storage protein, Amylase, Soybean Proteins, Digestion, Plant lipid transfer proteins, medicine.drug |
| الوصف: | Background: The 2S albumins are a group of storage proteins that occur widely in seeds of dicotyledonous plants. The widespread distribution and stability to digestion of allergenic 2S albumins raise the question of why some members of this family present in important food sources, such as soybean, are not regarded as major allergens. Methods: The pepsinolytic stability of two 2S albumins from soybean seed was determined using simulated gastric fluid. Using a new protein microarray system, IgE binding to these soybean 2S albumins was studied with the sera from 23 European individuals allergic to soybean. In order to validate the microarray result, two of the sera were selected and further tested using the micro-ELISA and UniCAP system. Results: Both albumins exhibited high stability to digestion similar to other allergenic members of the 2S albumin, trypsin/amylase inhibitor and lipid transfer protein superfamily. None of the patients was found to have IgE specific to soybean 2S albumins by the microarray system, and this result was in agreement with the results from the micro-ELISA and UniCAP system.Conclusions: The results from microarray, micro-ELISA and UniCAP system suggested that the 2S albumins from soybean are not major allergens within the patient population analyzed. |
| تدمد: | 1423-0097 1018-2438 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::e2cb0763cc1bba65c88eed70d3da56c1Test https://doi.org/10.1159/000094535Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........e2cb0763cc1bba65c88eed70d3da56c1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14230097 10182438 |
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