التفاصيل البيبلوغرافية
| العنوان: |
Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway |
| المؤلفون: |
Clemente, Claudia, Johnson, Nicholas, Ouyang, Xiaodan, Popin, Rafael, Dall'Angelo, Sergio, Wahlsten, Matti, Jokela, Jouni, Colombano, Alessandro, Nardone, Brunello, Fewer, David P., Houssen, Wael E. |
| المساهمون: |
Institute of Biotechnology, Department of Food and Nutrition, Department of Microbiology, Faculty of Agriculture and Forestry, Cyanobacteria research, Helsinki Institute of Sustainability Science (HELSUS), Microbial Natural Products |
| بيانات النشر: |
Royal Society of Chemistry |
| سنة النشر: |
2022 |
| المجموعة: |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: |
DRUG DISCOVERY, CYCLIC PEPTIDE, MACROCYCLASE, HETEROCYCLIZATION, TRYPTOPHAN, PROTEINS, ENZYMES, ENABLES, Biochemistry, cell and molecular biology |
| الوصف: |
Cyanobactins are linear and cyclic post-translationally modified peptides. Here we show that the prenyl-D-Arg-containing autum-nalamide A is a member of the cyanobactin family. Biochemical assays demonstrate that the AutF prenyltransferase targets the guanidinium moiety in arginine and homoarginine and is a useful tool for biotechnological applications. ; Peer reviewed |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
This project was supported by a fellowship grant from the EPSRC (No. EP/S027246/1, W.E.H.), a training grant from the BBSRC (BB/V509206/1, W. E. H. and S. D.), the Novo Nordisk Foundation (18OC0034838, D. P. F.) and the NordForsk NCoE program ``NordAqua'' (Project Number 82845, D. P. F.). C. C. is funded by a PhD studentship from University of Aberdeen. N. J. is funded by the IBioIC CTP PhD programme. R. V. P. was funded by the Doctoral Programme in Microbiology and Biotechnology of the University of Helsinki. X. O. was funded by the China Scholarship Council (Grant 201906150148). We are grateful to Professor James Naismith (University of Oxford) for sharing the construct used to express the macrocyclase PCY1. We thank Dr Huanting Liu (University of St Andrews) for sharing the pEHISTEV-SUMO vector.; Clemente , C , Johnson , N , Ouyang , X , Popin , R , Dall'Angelo , S , Wahlsten , M , Jokela , J , Colombano , A , Nardone , B , Fewer , D P & Houssen , W E 2022 , ' Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway ' , Chemical Communications , vol. 58 , no. 86 , pp. 12054-12057 . https://doi.org/10.1039/d2cc01799gTest; ORCID: /0000-0003-3978-4845/work/123135547; ORCID: /0000-0001-5096-3575/work/123136680; ORCID: /0000-0002-4107-1695/work/123138419; ORCID: /0000-0003-0427-0326/work/123143232; http://hdl.handle.net/10138/350678Test; 3b58f94b-ecb7-4af4-af19-d4b0750e3f24; 000863636300001 |
| الإتاحة: |
http://hdl.handle.net/10138/350678Test |
| حقوق: |
cc_by ; info:eu-repo/semantics/openAccess ; openAccess |
| رقم الانضمام: |
edsbas.618B3234 |
| قاعدة البيانات: |
BASE |
