التفاصيل البيبلوغرافية
| العنوان: |
Activation-induced cytidine deaminase-mediated hypermutation in the DT40 cell line. |
| المؤلفون: |
Hiroshi Arakawa1, Jean-Marie Buerstedde1 |
| المصدر: |
Philosophical Transactions of the Royal Society B: Biological Sciences. Mar2009, Vol. 364 Issue 1517, p639-644. 6p. |
| مصطلحات موضوعية: |
*CELL lines, *B cells, *CELL culture, *LYMPHOCYTES |
| مستخلص: |
Depending on the species and the developmental stage of B cells, activation-induced cytidine deaminase (AID) triggers immunoglobulin (Ig) gene diversification by gene conversion, hypermutation or switch recombination. The bursal B cell line DT40 usually diversifies its rearranged Ig light chain (IgL) gene by gene conversion, but disruption of the RAD51 gene paralogues or deletion of the ψV conversion donors induces hypermutation. Although not all aspects of somatic hypermutation can be studied in DT40, the compact size of the chicken IgL locus and the ability to modify the genome by targeted integration are powerful experimental advantages. We review here how the studies in DT40 contributed to understanding how AID initiates Ig gene diversification and how AID-induced uracils are subsequently processed by uracil DNA glycosylase, proliferating cell nuclear antigens and error-prone polymerases. We also discuss the on-going research on the Ig locus specificity of hypermutation and the possibility of using hypermutation for the artificial evolution of proteins and regulatory sequences in DT40. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
Full Text Finder