Different roles for the Fc epsilon RI gamma chain as a function of the receptor context
| العنوان: | Different roles for the Fc epsilon RI gamma chain as a function of the receptor context |
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| المؤلفون: | Rossella Paolini, Eric Vivier, V Renard, Bernard Malissen, Kenichi Ochiai, M.-H. Jouvin, Jean-Pierre Kinet |
| المصدر: | The Journal of Experimental Medicine |
| بيانات النشر: | Rockefeller University Press, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Interleukin 2, Macromolecular Substances, Receptors, Antigen, T-Cell, alpha-beta, CD3, T cell, Molecular Sequence Data, Immunology, In Vitro Techniques, Biology, Transfection, CD3 Complex, Mice, medicine, Animals, Immunology and Allergy, Electrophoresis, Gel, Two-Dimensional, Amino Acid Sequence, Phosphotyrosine, Receptor, Receptors, IgE, T-cell receptor, Articles, Phosphoproteins, Molecular biology, medicine.anatomical_structure, biology.protein, Interleukin-2, Tyrosine, Phosphorylation, Signal transduction, Signal Transduction, medicine.drug |
| الوصف: | The high affinity immunoglobulin E receptor (Fc epsilon RI) and the B and T cell antigen receptors (TCR) are multimeric complexes containing subunits with cytoplasmic antigen recognition activation motifs (ARAMs). The presence of multiple motifs may be a way to amplify a single signal or provide independent activation modules. Here we have compared the signaling capacity of the same Fc epsilon RI gamma motif in the context of two different receptors, Fc epsilon RI and TCR/CD3, simultaneously reconstituted on the surface of the same zeta-deficient T cell line. Both reconstituted receptors mediate early (phosphorylation) and late (interleukin [IL]-2 release) signals. Mutation of the two tyrosine residues of ARAM gamma alters early signaling by both receptors, but the set of substrates phosphorylated via ARAM gamma is different for each receptor and is thus dependent on the receptor context. Furthermore, the mutations prevent Fc epsilon RI- but not TCR/CD3-mediated IL-2 release. These data demonstrate that ARAM gamma is necessary for allowing both receptors to phosphorylate the complete set of substrates, and that the CD3 complex, unlike the Fc epsilon RI beta chain, contains activation modules capable of compensating for the absence of a functional ARAM gamma in generating late signals such as IL-2 release. |
| تدمد: | 1540-9538 0022-1007 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b83e95f968f14c1fc0d30d00a5942eecTest https://doi.org/10.1084/jem.181.1.247Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b83e95f968f14c1fc0d30d00a5942eec |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15409538 00221007 |
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