DNA Methylation Maintains Allele-specific KIR Gene Expression in Human Natural Killer Cells
| العنوان: | DNA Methylation Maintains Allele-specific KIR Gene Expression in Human Natural Killer Cells |
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| المؤلفون: | Brian E. Mace, C. Andrew Stewart, Zoya B. Kurago, Charles T. Lutz, Maureen P. Martin, Mary Carrington, Huei Wei Chan, John Trowsdale, Michael J. Wilson |
| المصدر: | The Journal of Experimental Medicine |
| بيانات النشر: | Rockefeller University Press, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Immunology, killer inhibitory receptor, Gene Expression, chemical and pharmacologic phenomena, Major histocompatibility complex, Article, Cell Line, KIR2DL4, Receptors, KIR, otorhinolaryngologic diseases, Humans, Immunology and Allergy, Receptors, Immunologic, Gene, Genetics, Regulation of gene expression, DNA methylation, biology, Histocompatibility Antigens Class I, Receptors, KIR3DL1, Receptors, KIR3DL2, gene expression regulation, Molecular biology, Clone Cells, Killer Cells, Natural, Receptors, KIR2DL4, KIR3DL2, alleles, biology.protein, KIR3DL1, DNA hypomethylation |
| الوصف: | Killer immunoglobulin-like receptors (KIR) bind self–major histocompatibility complex class I molecules, allowing natural killer (NK) cells to recognize aberrant cells that have down-regulated class I. NK cells express variable numbers and combinations of highly homologous clonally restricted KIR genes, but uniformly express KIR2DL4. We show that NK clones express both 2DL4 alleles and either one or both alleles of the clonally restricted KIR 3DL1 and 3DL2 genes. Despite allele-independent expression, 3DL1 alleles differed in the core promoter by only one or two nucleotides. Allele-specific 3DL1 gene expression correlated with promoter and 5′ gene DNA hypomethylation in NK cells in vitro and in vivo. The DNA methylase inhibitor, 5-aza-2′-deoxycytidine, induced KIR DNA hypomethylation and heterogeneous expression of multiple KIR genes. Thus, NK cells use DNA methylation to maintain clonally restricted expression of highly homologous KIR genes and alleles. |
| تدمد: | 1540-9538 0022-1007 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9c936637564338cfa9e371d1e1ba389Test https://doi.org/10.1084/jem.20021127Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e9c936637564338cfa9e371d1e1ba389 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15409538 00221007 |
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