دورية أكاديمية
Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A
| العنوان: | Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A |
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| المؤلفون: | Molli, Poonam R., Li, Da-Qiang, Bagheri-Yarmand, Rozita, Pakala, Suresh B., Katayama, Hiroshi, Sen, Subrata, Iyer, Jyoti, Chernoff, Jonathan, Tsai, Ming-Ying, Nair, Sujit S., Kumar, Rakesh |
| المصدر: | Journal of Cell Biology ; volume 190, issue 1, page 101-114 ; ISSN 1540-8140 0021-9525 |
| بيانات النشر: | Rockefeller University Press |
| سنة النشر: | 2010 |
| الوصف: | Here we provide evidence in support of an inherent role for Arpc1b, a component of the Arp2/3 complex, in regulation of mitosis and demonstrate that its depletion inhibits Aurora A activation at the centrosome and impairs the ability of mammalian cells to enter mitosis. We discovered that Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on threonine 21, and expression of Arpc1b but not a nonphosphorylatable Arpc1b mutant in mammalian cells leads to Aurora A kinase activation and abnormal centrosome amplification in a Pak1-independent manner. Together, these findings reveal a new function for Arpc1b in centrosomal homeostasis. Arpc1b is both a physiological activator and substrate of Aurora A kinase and these interactions help to maintain mitotic integrity in mammalian cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1083/jcb.200908050 |
| الإتاحة: | https://doi.org/10.1083/jcb.200908050Test https://rupress.org/jcb/article-pdf/190/1/101/1473435/jcb_200908050.pdfTest |
| رقم الانضمام: | edsbas.818C5BE7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1083/jcb.200908050 |
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