Signal recognition particle RNA localization within the nucleolus differs from the classical sites of ribosome synthesis
| العنوان: | Signal recognition particle RNA localization within the nucleolus differs from the classical sites of ribosome synthesis |
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| المؤلفون: | Laura B Lewandowski, Joan C. Ritland Politz, Thoru Pederson |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | Rockefeller University Press, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Dense fibrillar component, Chromosomal Proteins, Non-Histone, Nucleolus, Recombinant Fusion Proteins, 5.8S ribosomal RNA, Biology, environment and public health, Ribosome, Article, 03 medical and health sciences, nucleolus, signal recognition particle, SRP RNA, ribosome synthesis, peptide nucleic acids, Nucleolus Organizer Region, RNA polymerase I, Animals, Humans, Signal recognition particle RNA, Cells, Cultured, In Situ Hybridization, 030304 developmental biology, Fibrillarin, 0303 health sciences, 030302 biochemistry & molecular biology, RNA, Cell Biology, Fibroblasts, Molecular biology, Rats, Cell biology, Ribonucleoproteins, Oligonucleotide Probes, Pol1 Transcription Initiation Complex Proteins, Ribosomes, Signal Recognition Particle, Cell Nucleolus |
| الوصف: | The nucleolus is the site of ribosome biosynthesis, but is now known to have other functions as well. In the present study we have investigated how the distribution of signal recognition particle (SRP) RNA within the nucleolus relates to the known sites of ribosomal RNA synthesis, processing, and nascent ribosome assembly (i.e., the fibrillar centers, the dense fibrillar component (DFC), and the granular component). Very little SRP RNA was detected in fibrillar centers or the DFC of the nucleolus, as defined by the RNA polymerase I–specific upstream binding factor and the protein fibrillarin, respectively. Some SRP RNA was present in the granular component, as marked by the protein B23, indicating a possible interaction with ribosomal subunits at a later stage of maturation. However, a substantial portion of SRP RNA was also detected in regions of the nucleolus where neither B23, UBF, or fibrillarin were concentrated. Dual probe in situ hybridization experiments confirmed that a significant fraction of nucleolar SRP RNA was not spatially coincident with 28S ribosomal RNA. These results demonstrate that SRP RNA concentrates in an intranucleolar location other than the classical stations of ribosome biosynthesis, suggesting that there may be nucleolar regions that are specialized for other functions. |
| تدمد: | 1540-8140 0021-9525 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9179c22402fa8267b5d32ec5686137d6Test https://doi.org/10.1083/jcb.200208037Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9179c22402fa8267b5d32ec5686137d6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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