دورية أكاديمية
Systematic characterization of the conformation and dynamics of budding yeast chromosome XII
| العنوان: | Systematic characterization of the conformation and dynamics of budding yeast chromosome XII |
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| المؤلفون: | Albert, Benjamin, Mathon, Julien, Shukla, Ashutosh, Saad, Hicham, Normand, Christophe, Léger-Silvestre, Isabelle, Villa, David, Kamgoué, Alain, Mozziconacci, Julien, Wong, Hua, Zimmer, Christophe, Bhargava, Purnima, Bancaud, Aurélien, Gadal, Olivier |
| المساهمون: | Laboratoire de biologie moléculaire eucaryote (LBME), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Council of Scientific and Industrial Research India (CSIR), Laboratoire de Physique Théorique de la Matière Condensée (LPTMC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), GDR 3536 Architecture et Dynamique Nucléaires (ADN), Université Pierre et Marie Curie - Paris 6 (UPMC), Imagerie et Modélisation, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), This work was supported by an ATIP-plus grant from Centre National de la Recherche Scientifique, by the Agence Nationale de la Recherche (Nucleopol, Ribeuc, and program JCJC), Jeune équipe from FRM, and University Paul Sabatier (a fellowship for J. Mathon). This work is a French-Indian collaborative effort funded by Indo-French Centre for the Promotion of Advanced Research (IFCPAR) Project 4103 between the laboratories of Dr. Bhargava and Dr. Gadal., ANR-08-PCVI-0036,NUCLEOPOL,Integrated structural biology of rDNA transcription(2008), ANR-06-BLAN-0037,RIBEUC,Ribosome biogenesis in eukaryotes: mechanisms and connections with other pathways(2006) |
| المصدر: | ISSN: 0021-9525. |
| بيانات النشر: | HAL CCSD Rockefeller University Press |
| سنة النشر: | 2013 |
| المجموعة: | Université Toulouse III - Paul Sabatier: HAL-UPS |
| مصطلحات موضوعية: | [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] |
| الوصف: | International audience ; Chromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII carries the ribosomal DNA (rDNA) that defines the nucleolus, a major hallmark of nuclear organization. We determined intranuclear positions of 15 loci distributed every ~100 kb along the chromosome, and investigated their motion over broad time scales (0.2-400 s). Loci positions and motions, except for the rDNA, were consistent with a computational model of chromosomes based on tethered polymers and with the Rouse model from polymer physics, respectively. Furthermore, rapamycin-dependent transcriptional reprogramming of the genome only marginally affected the chromosome XII internal large-scale organization. Our comprehensive investigation of chromosome XII is thus in agreement with recent studies and models in which long-range architecture is largely determined by the physical principles of tethered polymers and volume exclusion. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/23878273; hal-01682609; https://hal.science/hal-01682609Test; https://hal.science/hal-01682609v2/documentTest; https://hal.science/hal-01682609v2/file/201.full.pdfTest; PUBMED: 23878273; PUBMEDCENTRAL: PMC3718979 |
| DOI: | 10.1083/jcb.201208186 |
| الإتاحة: | https://doi.org/10.1083/jcb.201208186Test https://hal.science/hal-01682609Test https://hal.science/hal-01682609v2/documentTest https://hal.science/hal-01682609v2/file/201.full.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by-nc-saTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.44AA2728 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1083/jcb.201208186 |
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