دورية أكاديمية
Regulation, Integrase-Dependent Excision, and Horizontal Transfer of Genomic Islands in Legionella pneumophila
| العنوان: | Regulation, Integrase-Dependent Excision, and Horizontal Transfer of Genomic Islands in Legionella pneumophila |
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| المؤلفون: | Lautner, Monika, Schunder, Eva, Herrmann, Vroni, Heuner, Klaus |
| بيانات النشر: | Robert Koch-Institut |
| سنة النشر: | 2013 |
| المجموعة: | Robert Koch Institute: Publications |
| مصطلحات موضوعية: | Humans, Chromosomes Bacterial, Recombination Genetic, Acanthamoeba castellanii/microbiology, Bacterial Secretion Systems/genetics, Conjugation Genetic, Gene Transfer Horizontal, Genomic Islands, Integrases/metabolism, Legionella pneumophila/genetics, Legionella pneumophila/metabolism, Plasmids, 610 Medizin, ddc:610 |
| الوصف: | Legionella pneumophila is a Gram-negative freshwater agent which multiplies in specialized nutrient-rich vacuoles of amoebae. When replicating in human alveolar macrophages, Legionella can cause Legionnaires' disease. Recently, we identified a new type of conjugation/type IVA secretion system (T4ASS) in L. pneumophila Corby (named trb-tra). Analogous versions of trb-tra are localized on the genomic islands Trb-1 and Trb-2. Both can exist as an episomal circular form, and Trb-1 can be transferred horizontally to other Legionella strains by conjugation. In our current work, we discovered the importance of a site-specific integrase (Int-1, lpc2818) for the excision and conjugation process of Trb-1. Furthermore, we identified the genes lvrRABC (lpc2813 to lpc2816) to be involved in the regulation of Trb-1 excision. In addition, we demonstrated for the first time that a Legionella genomic island (LGI) of L. pneumophila Corby (LpcGI-2) encodes a functional type IV secretion system. The island can be transferred horizontally by conjugation and is integrated site specifically into the genome of the transconjugants. LpcGI-2 generates three different episomal forms. The predominant episomal form, form A, is generated integrase dependently (Lpc1833) and transferred by conjugation in a pilT-dependent manner. Therefore, the genomic islands Trb-1 and LpcGI-2 should be classified as integrative and conjugative elements (ICEs). Coculture studies of L. pneumophila wild-type and mutant strains revealed that the int-1 and lvrRABC genes (located on Trb-1) as well as lpc1833 and pilT (located on LpcGI-2) do not influence the in vivo fitness of L. pneumophila in Acanthamoeba castellanii. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://edoc.rki.de/oa/articles/reMQcABga5oxo/PDF/20gT6pwMkjQQ.pdfTest; http://edoc.rki.de/176904/1637Test; urn:nbn:de:0257-10032395; http://dx.doi.org/10.25646/1562Test |
| DOI: | 10.1128/JB.01739-12 |
| DOI: | 10.25646/1562 |
| الإتاحة: | https://doi.org/10.1128/JB.01739-12Test https://doi.org/10.25646/1562Test http://edoc.rki.de/oa/articles/reMQcABga5oxo/PDF/20gT6pwMkjQQ.pdfTest http://edoc.rki.de/176904/1637Test https://nbn-resolving.org/urn:nbn:de:0257-10032395Test |
| رقم الانضمام: | edsbas.ECCE3A42 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/JB.01739-12 |
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