Low Annexin A1 Level in HTLV-1 Infected Patients is a Potential Biomarker for the Clinical Progression and Diagnosis of HAM/TSP
| العنوان: | Low Annexin A1 Level in HTLV-1 Infected Patients is a Potential Biomarker for the Clinical Progression and Diagnosis of HAM/TSP |
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| المؤلفون: | Ricardo Ishak, Rodrigo Arcoverde Cerveira, Maísa Silva de Sousa, Carlos Araújo da Costa, Cláudia M. Rodrigues, Antonio Carlos Rosário Vallinoto, Luiz Ricardo Goulart, Ednelza da Silva Graça Amoras, Maria Alice Freitas Queiroz, Bárbara Brasil Santana |
| المصدر: | BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-8 (2021) BMC Infectious Diseases |
| بيانات النشر: | Research Square Platform LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, endocrine system, Asymptomatic, Sensitivity and Specificity, Serology, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, Annexin, immune system diseases, hemic and lymphatic diseases, Gene expression, Tropical spastic paraparesis, Medicine, Humans, lcsh:RC109-216, Annexin A1, Human T-lymphotropic virus 1, business.industry, virus diseases, Biomarker, Middle Aged, Viral Load, HTLV, medicine.disease, Prognosis, Paraparesis, Tropical Spastic, 030104 developmental biology, Infectious Diseases, Real-time polymerase chain reaction, ROC Curve, 030220 oncology & carcinogenesis, Immunology, Disease Progression, Biomarker (medicine), Female, medicine.symptom, business, HAM/TSP, Infection, Biomarkers, Research Article |
| الوصف: | Background Human T-lymphotropic virus 1 (HTLV-1) is etiologically associated with the chronic inflammatory neurodegenerative disease HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) Annexin A1 (AnxA1) is an anti-inflammatory protein with proposed neuroprotective and anti-neuroinflammatory functions. We hypothesized that ANXA1 gene expression may be dysregulated in HTLV-1-infected HAM/TSP patients. Methods This study involved 37 individuals infected with HTLV-1, including 21 asymptomatic (AS) carriers and 16 with HAM/TSP, and a control group of 30 individuals negative for HTLV-1 and HTLV-2. For AS HTLV-1-positive and HAM/TSP patients, ANXA1 and formyl peptide receptor (FPR1, FPR2 and FPR3) expression and HTLV-1 proviral load (PVL) in peripheral blood cells were evaluated by real-time quantitative PCR (qPCR), and plasma AnxA1 levels were determined by enzyme-linked immunosorbent assay (ELISA). Results ANXA1 gene expression was increased in the AS group compared with the HAM/TSP and control groups, but the differences were not statistically significant. FPR1 gene expression was higher in patients with HTLV-1 than in controls (AS, p = 0.0032; HAM/TSP, p p = 0.0045), and PVL was higher in patients with HAM/TSP than in AS individuals (p = 0.0162). The use of a combined ROC curve using Annexin 1 levels and proviral load significantly increased the sensitivity and specificity to predict progression to HAM/TSP (AUC = 0.851 and AUC = 0.937, respectively, to AUC = 1000). Conclusions Our results suggest that AnxA1 may be dysregulated in HAM/TSP patients. Serological detection of AnxA1 in association with proviral load may provide a prognostic biomarker for HTLV-1-associated neurodegenerative disease. |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25ebb978c058f4d4d298ed76cf36fc89Test https://doi.org/10.21203/rs.3.rs-34184/v1Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....25ebb978c058f4d4d298ed76cf36fc89 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |