دورية أكاديمية
Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold
العنوان: | Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold |
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المؤلفون: | Samaneh Khazaei, Masoud Soleimani, Zohreh Hojati, Seyyed hossein Ahmaditafti |
المصدر: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, Vol 15, Iss 5, Pp 368-377 (2021) |
بيانات النشر: | Qom University of Medical Sciences, 2021. |
سنة النشر: | 2021 |
المجموعة: | LCC:Medicine (General) |
مصطلحات موضوعية: | cardiovascular diseases, mesenchymal stem cells, mir-1, organ culture techniques, cell differentiation, Medicine (General), R5-920 |
الوصف: | Background and Objectives: Cardiovascular disease is one of the leading causes of death worldwide. Mesenchymal stem cells (MSCs) are one of the most common sources of cell-based therapies in heart regeneration. There are several approaches to differentiate MSCs into cardiac-like cells, such as genetic modification. In addition, using of 3D culture, such as hydrogels, increases the efficiency of differentiation. Methods: In the present study, lentiviruses containing microRNA 1 (miR- 1) and myocardium (Myocd) were co-transducted to mouse adipose-derived MSCs. Three days after, transduced MSCs were transferred to a hydrogel containing chitosan and collagen. After 21 days, the differentiation of encapsulated cells was evaluated. In this regard, the expression of cardiac markers such as NK2 homeobox 5 (Nkx2-5), GATA binding protein 4 (Gata4) and troponin T type 2 (Tnnt2) at the level of gene and protein were investigated. Results: The results of real-time quantitative polymerase chain reaction (qRT-PCR) and immunocytochemistry showed that co-induction of miR-1 and Myocd in MSCs followed by transfer to composite hydrogel increased the expression of cardiac markers. Conclusion: The use of 3D culture such as chitosan/collagen hydrogel improves the differentiation of MSCs and subsequently obtains more mature cells for use in cell-based regenerative medicine |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | Persian |
تدمد: | 1735-7799 2008-1375 |
العلاقة: | http://journal.muq.ac.ir/article-1-3193-en.htmlTest; https://doaj.org/toc/1735-7799Test; https://doaj.org/toc/2008-1375Test |
الوصول الحر: | https://doaj.org/article/6d6faa7f3bb8436f83dbe2103751b6ffTest |
رقم الانضمام: | edsdoj.6d6faa7f3bb8436f83dbe2103751b6ff |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 17357799 20081375 |
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