Chloroplast RNA-Binding Protein RBD1 Promotes Chilling Tolerance through 23S rRNA Processing in Arabidopsis
| العنوان: | Chloroplast RNA-Binding Protein RBD1 Promotes Chilling Tolerance through 23S rRNA Processing in Arabidopsis |
|---|---|
| المؤلفون: | Fen Yang, Shuai Wang, Leiyun Yang, Shu Wang, Bai Ge, Yi Wang, Jian Hua, Jian-Kang Zhu |
| المصدر: | Department of Horticulture & Landscape Architecture Faculty Publications PLoS Genetics, Vol 12, Iss 5, p e1006027 (2016) PLoS Genetics |
| بيانات النشر: | Purdue University, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Leaves, Cancer Research, Chloroplasts, Arabidopsis, RNA-binding proteins, RNA-binding protein, Plant Science, Biochemistry, 01 natural sciences, Gene Expression Regulation, Plant, Chloroplast localization, Protein biosynthesis, Arabidopsis thaliana, Photosynthesis, RNA Processing, Post-Transcriptional, Genetics (clinical), Genetics, Plant Anatomy, food and beverages, Plants, Adaptation, Physiological, Cell biology, Cold Temperature, Nucleic acids, Chloroplast, RNA, Ribosomal, 23S, Phenotypes, Phenotype, Ribonucleoproteins, Ribosomal RNA, Cellular Structures and Organelles, Cellular Types, Genome, Plant, Research Article, lcsh:QH426-470, Plant Cell Biology, Arabidopsis Thaliana, Brassica, Biology, Horticulture, 03 medical and health sciences, Extraction techniques, Model Organisms, Plant and Algal Models, Plant Cells, parasitic diseases, Non-coding RNA, Molecular Biology, Gene, Ecology, Evolution, Behavior and Systematics, Chloroplast nucleoid, Arabidopsis Proteins, fungi, Organisms, Biology and Life Sciences, Proteins, Genetics and Genomics, Cell Biology, biology.organism_classification, RNA extraction, Plant Leaves, Research and analysis methods, lcsh:Genetics, 030104 developmental biology, Mutation, RNA, Ribosomes, 010606 plant biology & botany |
| الوصف: | Plants have varying abilities to tolerate chilling (low but not freezing temperatures), and it is largely unknown how plants such as Arabidopsis thaliana achieve chilling tolerance. Here, we describe a genome-wide screen for genes important for chilling tolerance by their putative knockout mutants in Arabidopsis thaliana. Out of 11,000 T-DNA insertion mutant lines representing half of the genome, 54 lines associated with disruption of 49 genes had a drastic chilling sensitive phenotype. Sixteen of these genes encode proteins with chloroplast localization, suggesting a critical role of chloroplast function in chilling tolerance. Study of one of these proteins RBD1 with an RNA binding domain further reveals the importance of chloroplast translation in chilling tolerance. RBD1 is expressed in the green tissues and is localized in the chloroplast nucleoid. It binds directly to 23S rRNA and the binding is stronger under chilling than at normal growth temperatures. The rbd1 mutants are defective in generating mature 23S rRNAs and deficient in chloroplast protein synthesis especially under chilling conditions. Together, our study identifies RBD1 as a regulator of 23S rRNA processing and reveals the importance of chloroplast function especially protein translation in chilling tolerance. Author Summary Compared to cold acclimation (enhancement of freezing tolerance by a prior exposure to low non-freezing temperature), the tolerance mechanism to non-freezing chilling temperatures is not well understood. Here, we performed a genome-wide mutant screen for chilling sensitive phenotype and identified 49 candidate genes important for chilling tolerance in Arabidopsis. Among the proteins encoded by these 49 genes, 16 are annotated as having chloroplast localization, suggesting a critical role of chloroplast function in chilling tolerance. We further studied RBD1, one of the four RNA-binding proteins localized to chloroplast. RBD1 is only expressed in the green photosynthetic tissues and is localized to nucleoid of chloroplasts. Furthermore, RBD1 is found to be a regulator of 23S rRNA processing likely through direct binding to the precursor of 23S rRNA in a temperature dependent manner. Our study thus reveals the importance of chloroplast function especially protein translation in chilling tolerance at genome-wide scale and suggests an adaptive mechanism involving low temperature enhanced activities from proteins such as RBD1 in chilling tolerance. |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5b25932c37faac4676adb9be70d37b5Test http://docs.lib.purdue.edu/hlapubs/19Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d5b25932c37faac4676adb9be70d37b5 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |