Low Density Lipoproteins Promote Unstable Calcium Handling Accompanied by Reduced SERCA2 and Connexin-40 Expression in Cardiomyocytes
| العنوان: | Low Density Lipoproteins Promote Unstable Calcium Handling Accompanied by Reduced SERCA2 and Connexin-40 Expression in Cardiomyocytes |
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| المؤلفون: | Barriga M., Cal R., Cabello N., Llach A., Vallmitjana A., Benítez R., Badimon L., Cinca J., Llorente-Cortés V., Hove-Madsen L. |
| المصدر: | PLoS One r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname |
| بيانات النشر: | PUBLIC LIBRARY SCIENCE, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | lipid storage, Intracellular Space, heart muscle cell, SERCA2 protein, animal cell, protein localization, calcium signaling, Connexins, connexin 43, low density lipoprotein cholesterol, Western blotting, Cell Line, Sarcoplasmic Reticulum Calcium-Transporting ATPases, Mice, connexin 40, ryanodine receptor 2, calcium transport, calcium ion, Animals, Humans, controlled study, Myocytes, Cardiac, cell protein, protein expression, mouse, calcium cell level, nonhuman, messenger RNA, IP3RII protein, article, Cholesterol, LDL, protein function, IP3RI protein, molecular dynamics, unclassified drug, Lipoproteins, LDL, carrier protein, cell level, Gene Expression Regulation, real time polymerase chain reaction, Calcium, Cholesterol Esters, low density lipoprotein, protein determination |
| الوصف: | The damaging effects of high plasma levels of cholesterol in the cardiovascular system are widely known, but little attention has been paid to direct effects on cardiomyocyte function. We therefore aimed at testing the hypothesis that Low Density Lipoprotein (LDL) cholesterol affects calcium dynamics and signal propagation in cultured atrial myocytes. For this purpose, mRNA and protein expression levels were determined by real time PCR and western blot analysis, respectively, and intracellular calcium was visualized in fluo-4 loaded atrial HL-1 myocyte cultures subjected to field stimulation. At low stimulation frequencies all cultures had uniform calcium transients at all tested LDL concentrations. However, 500 µg LDL/mL maximally reduced the calcium transient amplitude by 43% from 0.30±0.04 to 0.17±0.02 (p |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=RECOLECTA___::3b6d9dbe54be35bd9a16891606a5e3afTest https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874928068&doi=10.1371/journal.pone.0058128&partnerID=40&md5=d3bcf692c671eb4f3e11bdad2b1d455eTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.RECOLECTA.....3b6d9dbe54be35bd9a16891606a5e3af |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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