Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells
| العنوان: | Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells |
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| المؤلفون: | María Luz Valero, Dolores Bernal, Manuel M. Sánchez del Pino, Fernando Cantalapiedra, Rafael Toledo, Carla Muñoz-Antoli, Alba Cortés, Maria Teresa Minguez, María Trelis, Antonio Marcilla, Javier Sotillo |
| المصدر: | Plos One r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) Centro de Investigación Principe Felipe (CIPF) r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) PLoS ONE PLoS ONE, Vol 7, Iss 9, p e45974 (2012) |
| بيانات النشر: | PUBLIC LIBRARY SCIENCE, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Proteomics, Fascioliasis, Science, Endocytic cycle, Helminth Infection, Soil-Transmitted Helminths, Exosomes, Biochemistry, Microbiology, Host-Parasite Interactions, Cell Line, Tumor, Echinostoma, Molecular Cell Biology, Parasitic Diseases, Animals, Humans, Secretion, Intestinal Mucosa, Biology, Echinostomiasis, Multidisciplinary, biology, Vesicle, Parasite Physiology, Proteins, Helminth Proteins, Immunogold labelling, Fasciola hepatica, biology.organism_classification, Microvesicles, Rats, Cell biology, Host-Pathogen Interaction, Infectious Diseases, Secretory protein, Small Molecules, Excretory system, Medicine, Protozoa, Parasitology, Membranes and Sorting, Zoology, Research Article, Helminthology, Neglected Tropical Diseases |
| الوصف: | The study of host-parasite interactions has increased considerably in the last decades, with many studies focusing on the identification of parasite molecules (i.e. surface or excretory/secretory proteins (ESP)) as potential targets for new specific treatments and/or diagnostic tools. In parallel, in the last few years there have been significant advances in the field of extracellular vesicles research. Among these vesicles, exosomes of endocytic origin, with a characteristic size ranging from 30-100 nm, carry several atypical secreted proteins in different organisms, including parasitic protozoa. Here, we present experimental evidence for the existence of exosome-like vesicles in parasitic helminths, specifically the trematodes Echinostoma caproni and Fasciola hepatica. These microvesicles are actively released by the parasites and are taken up by host cells. Trematode extracellular vesicles contain most of the proteins previously identified as components of ESP, as confirmed by proteomic, immunogold labeling and electron microscopy studies. In addition to parasitic proteins, we also identify host proteins in these structures. The existence of extracellular vesicles explains the secretion of atypical proteins in trematodes, and the demonstration of their uptake by host cells suggests an important role for these structures in host-parasite communication, as described for other infectious agents. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f88e49d33f4989eabb9c1d59cfd12699Test https://fundanet.iislafe.san.gva.es/publicaciones/ProdCientif/PublicacionFrw.aspx?id=1446Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f88e49d33f4989eabb9c1d59cfd12699 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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