Association of Promoter Methylation of VGF and PGP9.5 with Ovarian Cancer Progression
| العنوان: | Association of Promoter Methylation of VGF and PGP9.5 with Ovarian Cancer Progression |
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| المؤلفون: | Mariana, Brait, Leonel, Maldonado, Maartje G, Noordhuis, Maartje, Noordhuis, Shahnaz, Begum, Myriam, Loyo, Maria Luana, Poeta, Alvaro, Barbosa, Vito M, Fazio, Roberto, Angioli, Carla, Rabitti, Luigi, Marchionni, Pauline, de Graeff, Ate G J, van der Zee, G Bea A, Wisman, David, Sidransky, Mohammad O, Hoque |
| المساهمون: | Targeted Gynaecologic Oncology (TARGON), Guided Treatment in Optimal Selected Cancer Patients (GUTS) |
| المصدر: | PLoS ONE, Vol 8, Iss 9, p e70878 (2013) PLoS ONE PLoS ONE, 8(9):e70878. PUBLIC LIBRARY SCIENCE |
| بيانات النشر: | PUBLIC LIBRARY SCIENCE, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | endocrine system diseases, genetic association, polymerase chain reaction, lcsh:Medicine, cancer cell culture, Cohort Studies, Prostate cancer, 0302 clinical medicine, cell growth, cancer survival, Promoter Regions, Genetic, lcsh:Science, DNA METHYLATION, Ovarian Neoplasms, 0303 health sciences, Multidisciplinary, Reverse Transcriptase Polymerase Chain Reaction, article, ovary cancer, Methylation, Middle Aged, 3. Good health, PROSTATE-CANCER, Real-time polymerase chain reaction, 030220 oncology & carcinogenesis, DNA methylation, cancer grading, Azacitidine, Disease Progression, MULTIPLE GENES, Female, Ubiquitin Thiolesterase, VGF gene, medicine.drug, Research Article, survival rate, Adult, EXPRESSION, in vitro study, gene overexpression, overall survival, cancer specific survival, BLADDER-CANCER, PGP9 5 gene, Biology, gene frequency, Decitabine, Real-Time Polymerase Chain Reaction, cancer growth, 03 medical and health sciences, Breast cancer, promoter region, medicine, Humans, BREAST-CANCER, controlled study, human, Nerve Growth Factors, survival time, 030304 developmental biology, Aged, DNA Primers, Bladder cancer, Base Sequence, cancer staging, human cell, lcsh:R, PROFILES, medicine.disease, HYPERMETHYLATION, Molecular biology, major clinical study, clinical feature, 7 INGENIERÍA Y TECNOLOGÍA, TUMOR-SUPPRESSOR GENES, Cancer research, PROGNOSTIC-FACTOR, lcsh:Q, Ovarian cancer |
| الوصف: | Purpose: To elucidate the role of biological and clinical impact of aberrant promoter hypermethylation (PH) in ovarian cancer (OC).Experimental Design: PH of PGP9.5, HIC1, AIM1, APC, PAK3, MGMT, KIF1A, CCNA1, ESR1, SSBP2, GSTP1, FKBP4 and VGF were assessed by quantitative methylation specific PCR (QMSP) in a training set. We selected two genes (VGF and PGP9.5) for further QMSP analysis in a larger independent validation (IV) set with available clinical data. Biologic relevance of VGF gene was also evaluated.Results: PH frequency for PGP9.5 and VGF were 85% (316/372) and 43% (158/366) respectively in the IV set of samples while no PH was observed in controls. In 372 OC cases with available follow up, PGP9.5 and VGF PH were correlated with better patient survival [Hazard Ratios (HR) for overall survival (OS) were 0.59 (95% Confidence Intervals (CI) = 0.42-0.84, p = 0.004), and 0.73 (95% CI = 0.55-0.97, p = 0.028) respectively, and for disease specific survival (DSS) were 0.57 (95% CI 0.39-0.82, p = 0.003) and 0.72 (95% CI 0.54-0.96, p = 0.027). In multivariate analysis, VGF PH remained an independent prognostic factor for OS (HR 0.61, 95% CI 0.43-0.86, p Conclusions: Our results indicate that VGF and PGP9.5 PH are potential biomarkers for ovarian carcinoma. Confirmatory cohorts with longitudinal follow-up are required in future studies to define the clinical impact of VGF and PGP9.5 PH before clinical application. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69df9d698e3a96612bb0b9bd89558dc9Test https://research.rug.nl/en/publications/d8bb8e87-0391-4cc6-8bf2-872d67eece0cTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....69df9d698e3a96612bb0b9bd89558dc9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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