Isolation, characterization and potential role in beta cell-endothelium cross-talk of extracellular vesicles released from human pancreatic islets
| العنوان: | Isolation, characterization and potential role in beta cell-endothelium cross-talk of extracellular vesicles released from human pancreatic islets |
|---|---|
| المؤلفون: | Vincenzo Cantaluppi, Giovanni Camussi, Luigi Biancone, Ciro Tetta, Renato Romagnoli, Michela De Lena, Silvia Beltramo, Raffaella Melzi, Lorenzo Piemonti, Rita Nano, Mauro Salizzoni, Federico Figliolini |
| المساهمون: | Figliolini, Federico, Cantaluppi, Vincenzo, De Lena, Michela, Beltramo, Silvia, Romagnoli, Renato, Salizzoni, Mauro, Melzi, Raffaella, Nano, Rita, Piemonti, Lorenzo, Tetta, Ciro, Biancone, Luigi, Camussi, Giovanni, Pathology/molecular and cellular medicine |
| المصدر: | PLoS ONE, Vol 9, Iss 7, p e102521 (2014) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Angiogenesis, Physiology, Cell Communication, Insulin-Secreting Cells, Medicine and Health Sciences, Endothelial Cell, Multidisciplinary, geography.geographical_feature_category, Reverse Transcriptase Polymerase Chain Reaction, Research Support, Non-U.S. Gov't, Medicine (all), MicroRNA, Islet, Flow Cytometry, Blot, Vascular endothelial growth factor A, medicine.anatomical_structure, Transport Vesicle, Medicine, Beta cell, Anatomy, extracellular vesicles, Research Article, Human, Cell signaling, Cell Physiology, endocrine system, Science, Blotting, Western, Angiogenesis Inducing Agent, Endocrine System, Biology, Cellule beta pancreatiche, medicine, Journal Article, Humans, RNA, Messenger, Transport Vesicles, Pancreas, geography, Biochemistry, Genetics and Molecular Biology (all), Pancreatic islets, Biology and Life Sciences, Endothelial Cells, Cell Biology, Receptor Cross-Talk, Molecular biology, Transplantation, MicroRNAs, Agricultural and Biological Sciences (all), Insulin-Secreting Cell, Angiogenesis Inducing Agents, Developmental Biology |
| الوصف: | The cross-talk between beta cells and endothelium plays a key role in islet physiopathology and in the revascularization process after islet transplantation. However, the molecular mechanisms involved in this cross-talk are not fully elucidated. Extracellular vesicles (EVs) are secreted membrane nanoparticles involved in inter-cellular communication through the transfer of proteins and nucleic acids. The aims of this study were: 1) isolation and characterization of EVs from human islets; 2) evaluation of the pro-angiogenic effect of islet-derived EVs on human islet endothelial cells (IECs). EVs were isolated by ultracentrifugation from conditioned medium of human islets and characterized by nanotrack analysis (Nanosight), FACS, western blot, bioanalyzer, mRNA/microRNA RT-PCR array. On IECs, we evaluated EV-induced insulin mRNA transfer, proliferation, resistance to apoptosis, in vitro angiogenesis, migration, gene and protein profiling. EVs sized 236±54 nm, expressed different surface molecules and islet-specific proteins (insulin, C-peptide, GLP1R) and carried several mRNAs (VEGFa, eNOS) and microRNAs (miR-27b, miR-126, miR-130 and miR-296) involved in beta cell function, insulin secretion and angiogenesis. Purified EVs were internalized into IECs inducing insulin mRNA expression, protection from apoptosis and enhancement of angiogenesis. Human islets release biologically active EVs able to shuttle specific mRNAs and microRNAs (miRNAs) into target endothelial cells. These results suggest a putative role for islet-derived EVs in beta cell-endothelium cross-talk and in the neoangiogenesis process which is critical for engraftment of transplanted islets. © 2014 Figliolini et al. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52e1bf5835553ecbc7afc5700a40cd12Test https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25028931/?tool=EBITest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....52e1bf5835553ecbc7afc5700a40cd12 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|