Virulence and Pathogen Multiplication: A Serial Passage Experiment in the Hypervirulent Bacterial Insect-Pathogen Xenorhabdus nematophila
| العنوان: | Virulence and Pathogen Multiplication: A Serial Passage Experiment in the Hypervirulent Bacterial Insect-Pathogen Xenorhabdus nematophila |
|---|---|
| المؤلفون: | Vanya Emelianoff, Elodie Chapuis, Sylvie Pages, Jean-Baptiste Ferdy, Alain Givaudan |
| المساهمون: | Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut de Recherche pour le Développement (IRD), Ecologie microbienne des insectes et interactions hôte-pathogène (EMIP), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | PLoS ONE PLoS ONE, Public Library of Science, 2011, 6 (1), pp.e15872. ⟨10.1371/journal.pone.0015872⟩ Plos One 1 (6), e15872. (2011) PLoS ONE, Vol 6, Iss 1, p e15872 (2011) PLoS ONE, 2011, 6 (1), pp.e15872. ⟨10.1371/journal.pone.0015872⟩ |
| بيانات النشر: | Public Library of Science (PLoS), 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | SELECTION, Insecta, lcsh:Medicine, Xenorhabdus, Pathogenesis, BACTERIE, PHENOTYPE, Serial passage, CROISSANCE, Gram Negative, hypervirulent bacterial, Serial Passage, évolution, lcsh:Science, Pathogen, bactérie, 0303 health sciences, Multidisciplinary, biology, Microbial Growth and Development, xenorhabdus nematophila, Phenotype, Bacterial Pathogens, INSECTE, pathogen multiplication, RELATION ANIMAL-PATHOGENE, Host-Pathogen Interactions, bactérie pathogène, Research Article, Virulence, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Forms of Evolution, Regulon, Microbiology, 03 medical and health sciences, Virology, Animals, Microevolution, Biology, Microbial Pathogens, 030304 developmental biology, multiplication, Evolutionary Biology, 030306 microbiology, Host (biology), lcsh:R, fungi, biology.organism_classification, Organismal Evolution, virulence, MICROBIOLOGIE, Microbial Evolution, Virulence Factors and Mechanisms, BACTERIOSE, VIRULENCE, lcsh:Q, Zoology, Entomology, Bacteria |
| الوصف: | Article en Open Access; International audience; The trade-off hypothesis proposes that the evolution of pathogens' virulence is shaped by a link between virulence and contagiousness. This link is often assumed to come from the fact that pathogens are contagious only if they can reach high parasitic load in the infected host. In this paper we present an experimental test of the hypothesis that selection on fast replication can affect virulence. In a serial passage experiment, we selected 80 lines of the bacterial insect-pathogen Xenorhabdus nematophila to multiply fast in an artificial culture medium. This selection resulted in shortened lag phase in our selected bacteria. We then injected these bacteria into insects and observed an increase in virulence. This could be taken as a sign that virulence in Xenorhabdus is linked to fast multiplication. But we found, among the selected lineages, either no link or a positive correlation between lag duration and virulence: the most virulent bacteria were the last to start multiplying. We then surveyed phenotypes that are under the control of the flhDC super regulon, which has been shown to be involved in Xenorhabdus virulence. We found that, in one treatment, the flhDC regulon has evolved rapidly, but that the changes we observed were not connected to virulence. All together, these results indicate that virulence is, in Xenorhabdus as in many other pathogens, a multifactorial trait. Being able to grow fast is one way to be virulent. But other ways exist which renders the evolution of virulence hard to predict |
| وصف الملف: | application/pdf; text/pdf |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fee2f0fa68fdec978dc03db6e41aac1fTest https://doi.org/10.1371/journal.pone.0015872Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fee2f0fa68fdec978dc03db6e41aac1f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|