دورية أكاديمية
أعرض في EDS

A cis-acting diversification activator both necessary and sufficient for AID-mediated hypermutation.

التفاصيل البيبلوغرافية
العنوان: A cis-acting diversification activator both necessary and sufficient for AID-mediated hypermutation.
المؤلفون: Artem Blagodatski, Vera Batrak, Sabine Schmidl, Ulrike Schoetz, Randolph B Caldwell, Hiroshi Arakawa, Jean-Marie Buerstedde
المصدر: PLoS Genetics, Vol 5, Iss 1, p e1000332 (2009)
بيانات النشر: Public Library of Science (PLoS), 2009.
سنة النشر: 2009
المجموعة: LCC:Genetics
مصطلحات موضوعية: Genetics, QH426-470
الوصف: Hypermutation of the immunoglobulin (Ig) genes requires Activation Induced cytidine Deaminase (AID) and transcription, but it remains unclear why other transcribed genes of B cells do not mutate. We describe a reporter transgene crippled by hypermutation when inserted into or near the Ig light chain (IgL) locus of the DT40 B cell line yet stably expressed when inserted into other chromosomal positions. Step-wise deletions of the IgL locus revealed that a sequence extending for 9.8 kilobases downstream of the IgL transcription start site confers the hypermutation activity. This sequence, named DIVAC for diversification activator, efficiently activates hypermutation when inserted at non-Ig loci. The results significantly extend previously reported findings on AID-mediated gene diversification. They show by both deletion and insertion analyses that cis-acting sequences predispose neighboring transcription units to hypermutation.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1553-7390
1553-7404
العلاقة: http://europepmc.org/articles/PMC2607555?pdf=renderTest; https://doaj.org/toc/1553-7390Test; https://doaj.org/toc/1553-7404Test
DOI: 10.1371/journal.pgen.1000332
الوصول الحر: https://doaj.org/article/079156d0041a4c42acafaec995b75adaTest
رقم الانضمام: edsdoj.079156d0041a4c42acafaec995b75ada
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:15537390
15537404
DOI:10.1371/journal.pgen.1000332