دورية أكاديمية
Innate sensing of HIV-infected cells.
| العنوان: | Innate sensing of HIV-infected cells. |
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| المؤلفون: | Alice Lepelley, Stéphanie Louis, Marion Sourisseau, Helen K W Law, Julien Pothlichet, Clémentine Schilte, Laurence Chaperot, Joël Plumas, Richard E Randall, Mustapha Si-Tahar, Fabrizio Mammano, Matthew L Albert, Olivier Schwartz |
| المصدر: | PLoS Pathogens, Vol 7, Iss 2, p e1001284 (2011) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2011 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5 |
| الوصف: | Cell-free HIV-1 virions are poor stimulators of type I interferon (IFN) production. We examined here how HIV-infected cells are recognized by plasmacytoid dendritic cells (pDCs) and by other cells. We show that infected lymphocytes are more potent inducers of IFN than virions. There are target cell-type differences in the recognition of infected lymphocytes. In primary pDCs and pDC-like cells, recognition occurs in large part through TLR7, as demonstrated by the use of inhibitors and by TLR7 silencing. Donor cells expressing replication-defective viruses, carrying mutated reverse transcriptase, integrase or nucleocapsid proteins induced IFN production by target cells as potently as wild-type virus. In contrast, Env-deleted or fusion defective HIV-1 mutants were less efficient, suggesting that in addition to TLR7, cytoplasmic cellular sensors may also mediate sensing of infected cells. Furthermore, in a model of TLR7-negative cells, we demonstrate that the IRF3 pathway, through a process requiring access of incoming viral material to the cytoplasm, allows sensing of HIV-infected lymphocytes. Therefore, detection of HIV-infected lymphocytes occurs through both endosomal and cytoplasmic pathways. Characterization of the mechanisms of innate recognition of HIV-infected cells allows a better understanding of the pathogenic and exacerbated immunologic events associated with HIV infection. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1553-7366 1553-7374 |
| العلاقة: | http://europepmc.org/articles/PMC3040675?pdf=renderTest; https://doaj.org/toc/1553-7366Test; https://doaj.org/toc/1553-7374Test; https://doaj.org/article/cde2846b4a194f389796090e8dd7f10eTest |
| DOI: | 10.1371/journal.ppat.1001284 |
| الإتاحة: | https://doi.org/10.1371/journal.ppat.1001284Test https://doaj.org/article/cde2846b4a194f389796090e8dd7f10eTest |
| رقم الانضمام: | edsbas.1AC2CE9B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 15537366 15537374 |
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| DOI: | 10.1371/journal.ppat.1001284 |