دورية أكاديمية
The thermodynamics of Pr55Gag-RNA interaction regulate the assembly of HIV.
| العنوان: | The thermodynamics of Pr55Gag-RNA interaction regulate the assembly of HIV. |
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| المؤلفون: | Hanumant S Tanwar, Keith K Khoo, Megan Garvey, Lynne Waddington, Andrew Leis, Marcel Hijnen, Tony Velkov, Geoff J Dumsday, William J McKinstry, Johnson Mak |
| المصدر: | PLoS Pathogens, Vol 13, Iss 2, p e1006221 (2017) |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Immunologic diseases. Allergy LCC:Biology (General) |
| مصطلحات موضوعية: | Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5 |
| الوصف: | The interactions that occur during HIV Pr55Gag oligomerization and genomic RNA packaging are essential elements that facilitate HIV assembly. However, mechanistic details of these interactions are not clearly defined. Here, we overcome previous limitations in producing large quantities of full-length recombinant Pr55Gag that is required for isothermal titration calorimetry (ITC) studies, and we have revealed the thermodynamic properties of HIV assembly for the first time. Thermodynamic analysis showed that the binding between RNA and HIV Pr55Gag is an energetically favourable reaction (ΔG |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1553-7366 1553-7374 |
| العلاقة: | http://europepmc.org/articles/PMC5336307?pdf=renderTest; https://doaj.org/toc/1553-7366Test; https://doaj.org/toc/1553-7374Test |
| DOI: | 10.1371/journal.ppat.1006221 |
| الوصول الحر: | https://doaj.org/article/958df98a5bb8424bb081bb2b7192b201Test |
| رقم الانضمام: | edsdoj.958df98a5bb8424bb081bb2b7192b201 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15537366 15537374 |
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| DOI: | 10.1371/journal.ppat.1006221 |