A Comprehensive Analysis of the Phylogeny, Genomic Organization and Expression of Immunoglobulin Light Chain Genes in Alligator sinensis, an Endangered Reptile Species
| العنوان: | A Comprehensive Analysis of the Phylogeny, Genomic Organization and Expression of Immunoglobulin Light Chain Genes in Alligator sinensis, an Endangered Reptile Species |
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| المؤلفون: | Yan Lu, Liming Ren, Xiaobing Wu, Chenglin Zhang, Xifeng Wang, Haitang Han, Gang Cheng, Yaofeng Zhao |
| المصدر: | PLoS ONE, Vol 11, Iss 2, p e0147704 (2016) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Xenopus, Alligator, lcsh:Medicine, Bird Genomics, Genome, Database and Informatics Methods, lcsh:Science, Reptile Genomics, Phylogeny, Genomic organization, Genetics, Alligators and Crocodiles, Multidisciplinary, biology, Phylogenetic tree, Bird Genetics, Phylogenetic Analysis, Genomics, Animal Models, Genomic Databases, Vertebrates, Frogs, Genes, Immunoglobulin Light Chain, Research Article, Immunoglobulin gene, Locus (genetics), Research and Analysis Methods, Amphibians, 03 medical and health sciences, Model Organisms, Phylogenetics, biology.animal, Animals, Molecular Biology Techniques, Molecular Biology, Gene, Molecular Biology Assays and Analysis Techniques, Endangered Species, lcsh:R, Organisms, Biology and Life Sciences, Computational Biology, Genome Analysis, Biological Databases, 030104 developmental biology, Genetic Loci, Animal Genomics, lcsh:Q, Animal Genetics, Cloning |
| الوصف: | Crocodilians are evolutionarily distinct reptiles that are distantly related to lizards and are thought to be the closest relatives of birds. Compared with birds and mammals, few studies have investigated the Ig light chain of crocodilians. Here, employing an Alligator sinensis genomic bacterial artificial chromosome (BAC) library and available genome data, we characterized the genomic organization of the Alligator sinensis IgL gene loci. The Alligator sinensis has two IgL isotypes, λ and κ, the same as Anolis carolinensis. The Igλ locus contains 6 Cλ genes, each preceded by a Jλ gene, and 86 potentially functional Vλ genes upstream of (Jλ-Cλ)n. The Igκ locus contains a single Cκ gene, 6 Jκs and 62 functional Vκs. All VL genes are classified into a total of 31 families: 19 Vλ families and 12 Vκ families. Based on an analysis of the chromosomal location of the light chain genes among mammals, birds, lizards and frogs, the data further confirm that there are two IgL isotypes in the Alligator sinensis: Igλ and Igκ. By analyzing the cloned Igλ/κ cDNA, we identified a biased usage pattern of V families in the expressed Vλ and Vκ. An analysis of the junctions of the recombined VJ revealed the presence of N and P nucleotides in both expressed λ and κ sequences. Phylogenetic analysis of the V genes revealed V families shared by mammals, birds, reptiles and Xenopus, suggesting that these conserved V families are orthologous and have been retained during the evolution of IgL. Our data suggest that the Alligator sinensis IgL gene repertoire is highly diverse and complex and provide insight into immunoglobulin gene evolution in vertebrates. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff72023c98d493b3bcaa450baedb363eTest https://doi.org/10.1371/journal.pone.0147704Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ff72023c98d493b3bcaa450baedb363e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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