Endothelium Derived Nitric Oxide Synthase Negatively Regulates the PDGF-Survivin Pathway during Flow-Dependent Vascular Remodeling
| العنوان: | Endothelium Derived Nitric Oxide Synthase Negatively Regulates the PDGF-Survivin Pathway during Flow-Dependent Vascular Remodeling |
|---|---|
| المؤلفون: | Philip M. Bauer, William C. Sessa, Jun Yu, Dario C. Altieri, Xinbo Zhang, Yuanyuan Zhang, R. Daniel Rudic |
| المصدر: | PLoS ONE, Vol 7, Iss 2, p e31495 (2012) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Anatomy and Physiology, Vascular smooth muscle, Survivin, lcsh:Medicine, Apoptosis, Cardiovascular, Biochemistry, Mechanotransduction, Cellular, Muscle, Smooth, Vascular, Inhibitor of Apoptosis Proteins, Immunoenzyme Techniques, Mice, chemistry.chemical_compound, Enos, Molecular Cell Biology, Biomechanics, lcsh:Science, Musculoskeletal System, Cells, Cultured, Mice, Knockout, Platelet-Derived Growth Factor, Multidisciplinary, biology, Physics, Nitric Oxide Synthase Type III, Neurochemistry, Cell biology, Nitric oxide synthase, medicine.anatomical_structure, Medicine, Neurochemicals, Cellular Types, Platelet-derived growth factor receptor, Research Article, Signal Transduction, Protein Binding, Endothelium, Blotting, Western, Biophysics, Neovascularization, Physiologic, Nitric Oxide, Real-Time Polymerase Chain Reaction, Nitric oxide, medicine, Animals, RNA, Messenger, Biology, Cell Proliferation, lcsh:R, biology.organism_classification, Mice, Inbred C57BL, Repressor Proteins, chemistry, Immunology, biology.protein, Blood Vessels, lcsh:Q, Endothelium, Vascular, Neuroscience |
| الوصف: | Chronic alterations in blood flow initiate structural changes in vessel lumen caliber to normalize shear stress. The loss of endothelial derived nitric oxide synthase (eNOS) in mice promotes abnormal flow dependent vascular remodeling, thus uncoupling mechanotransduction from adaptive vascular remodeling. However, the mechanisms of how the loss of eNOS promotes abnormal remodeling are not known. Here we show that abnormal flow-dependent remodeling in eNOS knockout mice (eNOS (-/-)) is associated with activation of the platelet derived growth factor (PDGF) signaling pathway leading to the induction of the inhibitor of apoptosis, survivin. Interfering with PDGF signaling or survivin function corrects the abnormal remodeling seen in eNOS (-/-) mice. Moreover, nitric oxide (NO) negatively regulates PDGF driven survivin expression and cellular proliferation in cultured vascular smooth muscle cells. Collectively, our data suggests that eNOS negatively regulates the PDGF-survivin axis to maintain proportional flow-dependent luminal remodeling and vascular quiescence. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e46a56ac80eda3e21553f7a415866b46Test https://doi.org/10.1371/journal.pone.0031495Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e46a56ac80eda3e21553f7a415866b46 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|