Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport
| العنوان: | Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport |
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| المؤلفون: | Blaine Bisel, Joachim Seemann, Yanzhuang Wang, Jen Hsuan Wei, Danming Tang |
| المصدر: | PLoS ONE, Vol 3, Iss 2, p e1647 (2008) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Science, Golgi Apparatus, Mitosis, Biochemistry, Coat Protein Complex I, symbols.namesake, Cell Biology/Membranes and Sorting, Humans, Secretory pathway, Multidisciplinary, Chemistry, Vesicle, Golgi Matrix Proteins, Membrane Proteins, COPI, COP-Coated Vesicles, Golgi apparatus, Transport protein, Cell biology, Protein Transport, Golgi cisterna, symbols, Medicine, Research Article |
| الوصف: | The Golgi apparatus in mammalian cells is composed of flattened cisternae that are densely packed to form stacks. We have used the Golgi stacking protein GRASP65 as a tool to modify the stacking state of Golgi cisternae. We established an assay to measure protein transport to the cell surface in post-mitotic cells in which the Golgi was unstacked. Cells with an unstacked Golgi showed a higher transport rate compared to cells with stacked Golgi membranes. Vesicle budding from unstacked cisternae in vitro was significantly increased compared to stacked membranes. These results suggest that Golgi cisternal stacking can directly regulate vesicle formation and thus the rate of protein transport through the Golgi. The results further suggest that at the onset of mitosis, unstacking of cisternae allows extensive and rapid vesiculation of the Golgi in preparation for its subsequent partitioning. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89fe594dd60e38a9a58a28d255a56ecbTest https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18297130/?tool=EBITest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....89fe594dd60e38a9a58a28d255a56ecb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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