Diaphanous-Related Formin 2 and Profilin I Are Required for Gastrulation Cell Movements
| العنوان: | Diaphanous-Related Formin 2 and Profilin I Are Required for Gastrulation Cell Movements |
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| المؤلفون: | Meng-Ju Lin, Show-Wan Lou, Wei-Pang Huang, Shih-Lei Lai, Shyh-Jye Lee, Tun-Hao Chan |
| المصدر: | PLoS ONE PLoS ONE, Vol 3, Iss 10, p e3439 (2008) |
| بيانات النشر: | Public Library of Science (PLoS), 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Embryo, Nonmammalian, RHOA, lcsh:Medicine, Formins, Epiboly, Cell Biology/Cell Signaling, Profilins, Developmental Biology/Molecular Development, Cell Movement, Two-Hybrid System Techniques, Cell Biology/Cytoskeleton, Animals, Humans, lcsh:Science, cdc42 GTP-Binding Protein, Zebrafish, Developmental Biology/Embryology, Multidisciplinary, biology, Convergent extension, lcsh:R, Gastrulation, Zebrafish Proteins, biology.organism_classification, Molecular biology, Cell biology, Antisense Elements (Genetics), Cdc42 GTP-Binding Protein, Profilin, biology.protein, lcsh:Q, Carrier Proteins, rhoA GTP-Binding Protein, Protein Binding, Research Article |
| الوصف: | Intensive cellular movements occur during gastrulation. These cellular movements rely heavily on dynamic actin assembly. Rho with its associated proteins, including the Rho-activated formin, Diaphanous, are key regulators of actin assembly in cellular protrusion and migration. However, the function of Diaphanous in gastrulation cell movements remains unclear. To study the role of Diaphanous in gastrulation, we isolated a partial zebrafish diaphanous-related formin 2 (zdia2) clone with its N-terminal regulatory domains. The GTPase binding domain of zDia2 is highly conserved compared to its mammalian homologues. Using a yeast two-hybrid assay, we showed that zDia2 interacts with constitutively-active RhoA and Cdc42. The zdia2 mRNAs were ubiquitously expressed during early embryonic development in zebrafish as determined by RT-PCR and whole-mount in situ hybridization analyses. Knockdown of zdia2 by antisense morpholino oligonucleotides (MOs) blocked epiboly formation and convergent extension in a dose-dependent manner, whereas ectopic expression of a human mdia gene partially rescued these defects. Time-lapse recording further showed that bleb-like cellular processes of blastoderm marginal deep marginal cells and pseudopod-/filopod-like processes of prechordal plate cells and lateral cells were abolished in the zdia2 morphants. Furthermore, zDia2 acts cell-autonomously since transplanted zdia2-knockdown cells exhibited low protrusive activity with aberrant migration in wild type host embryos. Lastly, co-injection of antisense MOs of zdia2 and zebrafish profilin I (zpfn 1), but not zebrafish profilin II, resulted in a synergistic inhibition of gastrulation cell movements. These results suggest that zDia2 in conjunction with zPfn 1 are required for gastrulation cell movements in zebrafish. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::386e7da85602c61b4a0f5a4fdfddffdcTest https://doi.org/10.1371/journal.pone.0003439Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....386e7da85602c61b4a0f5a4fdfddffdc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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