Full-length genome sequences of porcine epidemic diarrhoea virus strain CV777; Use of NGS to analyse genomic and sub-genomic RNAs
| العنوان: | Full-length genome sequences of porcine epidemic diarrhoea virus strain CV777; Use of NGS to analyse genomic and sub-genomic RNAs |
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| المؤلفون: | Melcher, Ulrich, Rasmussen, Thomas Bruun, Boniotti, Maria Beatrice, Papetti, Alice, Grasland, Béatrice, Frossard, Jean-Pierre, Dastjerdi, Akbar, Hulst, Marcel, Hanke, Dennis, Pohlmann, Anne, Blome, Sandra, van der Poel, Wim H. M., Steinbach, Falko, Blanchard, Yannick, Lavazza, Antonio, Bøtner, Anette, Belsham, Graham J. |
| المصدر: | PLoS ONE, Vol 13, Iss 3, p e0193682 (2018) PLOS ONE PLoS ONE, 13(3) PLoS ONE Rasmussen, T B, Boniotti, M B, Papetti, A, Grasland, B, Frossard, J-P, Dastjerdi, A, Hulst, M, Hanke, D, Pohlmann, A, Blome, S, van der Poel, W H M, Steinbach, F, Blanchard, Y, Lavazza, A, Bøtner, A & Belsham, G J 2018, ' Full-length genome sequences of porcine epidemic diarrhoea virus strain CV777; Use of NGS to analyse genomic and sub-genomic RNAs ', P L o S One, vol. 13, no. 3, pp. e0193682 . https://doi.org/10.1371/journal.pone.0193682Test PLoS ONE 13 (2018) 3 |
| بيانات النشر: | Public Library of Science (PLoS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Swine, Cell Lines, lcsh:Medicine, medicine.disease_cause, Biochemistry, Genome, Database and Informatics Methods, Start codon, Coding region, Genomic library, DNA libraries, lcsh:Science, Phylogeny, Sequence Deletion, Coronavirus, Swine Diseases, Mammals, Genetics, Viral Genomics, Multidisciplinary, High-Throughput Nucleotide Sequencing, Eukaryota, Genomics, General Medicine, Virology & Molecular Biology, Nucleic acids, Vertebrates, RNA, Viral, Biological Cultures, Coronavirus Infections, General Agricultural and Biological Sciences, Sequence Analysis, Research Article, Animal Breeding & Genomics, Bioinformatics, Sequence analysis, 030106 microbiology, Genome, Viral, Microbial Genomics, Biology, Research and Analysis Methods, Microbiology, General Biochemistry, Genetics and Molecular Biology, Evolution, Molecular, Open Reading Frames, 03 medical and health sciences, SDG 3 - Good Health and Well-being, Sequence Motif Analysis, Virology, medicine, Life Science, Animals, Point Mutation, Fokkerij & Genomica, Vero Cells, Sequence Assembly Tools, Base Sequence, Sequence Analysis, RNA, Porcine epidemic diarrhea virus, lcsh:R, Organisms, Biology and Life Sciences, Computational Biology, DNA, Genome Analysis, Genomic Libraries, biology.organism_classification, Virologie & Moleculaire Biologie, 030104 developmental biology, Amniotes, lcsh:Q, Sequence Alignment, Reference genome |
| الوصف: | Porcine epidemic diarrhoea virus, strain CV777, was initially characterized in 1978 as the causative agent of a disease first identified in the UK in 1971. This coronavirus has been widely distributed among laboratories and has been passaged both within pigs and in cell culture. To determine the variability between different stocks of the PEDV strain CV777, sequencing of the full-length genome (ca. 28kb) has been performed in 6 different laboratories, using different protocols. Not surprisingly, each of the different full genome sequences were distinct from each other and from the reference sequence (Accession number AF353511) but they are >99% identical. Unique and shared differences between sequences were identified. The coding region for the surface-exposed spike protein showed the highest proportion of variability including both point mutations and small deletions. The predicted expression of the ORF3 gene product was more dramatically affected in three different variants of this virus through either loss of the initiation codon or gain of a premature termination codon. The genome of one isolate had a substantially rearranged 5´-terminal sequence. This rearrangement was validated through the analysis of sub-genomic mRNAs from infected cells. It is clearly important to know the features of the specific sample of CV777 being used for experimental studies. |
| وصف الملف: | text; application/octet-stream; application/pdf |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::716caa668ec6548af74ffe3c9623009dTest https://doi.org/10.1371/journal.pone.0193682Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....716caa668ec6548af74ffe3c9623009d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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