Antibiofilm Properties of Interfacially Active Lipase Immobilized Porous Polycaprolactam Prepared by LB Technique
| العنوان: | Antibiofilm Properties of Interfacially Active Lipase Immobilized Porous Polycaprolactam Prepared by LB Technique |
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| المؤلفون: | Veluchamy Prabhawathi, Ponnurengam Malliappan Sivakumar, Thulasinathan Boobalan, Mukesh Doble |
| المصدر: | PLoS ONE PLoS ONE, Vol 9, Iss 5, p e96152 (2014) |
| بيانات النشر: | Public Library of Science (PLoS), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Bacterial Diseases, Staphylococcus, Glycobiology, lcsh:Medicine, Biochemistry, chemistry.chemical_compound, Medicine and Health Sciences, Caprolactam, lcsh:Science, Candida, Fungal protein, Multidisciplinary, Ecology, biology, Organic Compounds, Biomaterial, Bacterial Pathogens, Chemistry, Infectious Diseases, Medical Microbiology, Physical Sciences, Porosity, Research Article, Biotechnology, Staphylococcus aureus, Biocompatibility, Immobilized enzyme, Materials Science, Carbohydrates, Triacylglycerol lipase, Microbiology, Microbial Ecology, Fungal Proteins, Biomaterials, Escherichia coli, Humans, Lipase, Microbial Pathogens, lcsh:R, Chemical Compounds, Biofilm, Biology and Life Sciences, Bacteriology, Enzymes, Immobilized, chemistry, Chemical engineering, Biofilms, biology.protein, bacterial protein, carbohydrate, glutaraldehyde, lactam derivative, lactate dehydrogenase, policapram, triacylglycerol lipase, unclassified drug, caprolactam, fungal protein, hydroxyprogesterone caproate, immobilized enzyme, 3T3 cell line, antibiofilm activity, antimicrobial activity, article, atomic force microscopy, bacterium colony, biocompatibility, biofilm, cell proliferation, cell wall, cross linking, cytotoxicity, enzyme activity, enzyme immobilization, enzyme stability, fluorescence microscopy, hydrophilicity, hydrophobicity, intermethod comparison, Langmuir Blodgett film, nonhuman, porosity, slimicidal activity, surface property, thermostability, drug effects, enzymology, human, physiology, lcsh:Q, Glutaraldehyde, Bacterial Biofilms |
| الوصف: | Porous biomaterial is the preferred implant due to the interconnectivity of the pores. Chances of infection due to biofilm are also high in these biomaterials because of the presence of pores. Although biofilm in implants contributes to 80% of human infections [1], there are no commercially available natural therapeutics against it. In the current study, glutaraldehyde cross linked lipase was transferred onto a activated porous polycaprolactam surface using Langmuir-Blodgett deposition technique, and its thermostability, slimicidal, antibacterial, biocompatibility and surface properties were studied. There was a 20% increase in the activity of the covalently crosslinked lipase when compared to its free form. This immobilized surface was thermostable and retained activity and stability until 100�C. There was a 2 and 7 times reduction in carbohydrate and 9 and 5 times reduction in biofilm protein of Staphylococcus aureus and Escherichia coli respectively on lipase immobilized polycaprolactam (LIP) when compared to uncoated polycaprolactam (UP). The number of live bacterial colonies on LIP was four times less than on UP. Lipase acted on the cell wall of the bacteria leading to its death, which was confirmed from AFM, fluorescence microscopic images and amount of lactate dehydrogenase released. LIP allowed proliferation of more than 90% of 3T3 cells indicating that it was biocompatible. The fact that LIP exhibits antimicrobial property at the air-water interface to hydrophobic as well as hydrophilic bacteria along with lack of cytotoxicity makes it an ideal biomaterial for biofilm prevention in implants. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::141cb8cd48a819a0b59afa76d8498b36Test https://doi.org/10.1371/journal.pone.0096152Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....141cb8cd48a819a0b59afa76d8498b36 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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