Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues
| العنوان: | Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues |
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| المؤلفون: | Vicente A. Resto, Monica M. Burdick, Venktesh S. Shirure, Grady E. Carlson, Douglas J. Goetz, Eric Martin, Ramiro Malgor |
| المساهمون: | Sperandio, Markus |
| المصدر: | PloS one, vol 12, iss 3 PLoS ONE PLoS ONE, Vol 12, Iss 3, p e0173747 (2017) |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Tissue Fixation, Papillary, lcsh:Medicine, Ligands, Biochemistry, Metastasis, Circulating tumor cell, Adenocarcinomas, Basic Cancer Research, Medicine and Health Sciences, Tissue Distribution, Mucinous, L-Selectin, lcsh:Science, Shear Stresses, Cancer, Microscopy, Multidisciplinary, biology, Physics, Classical Mechanics, Adenocarcinoma, Mucinous, Microspheres, Signet Ring Cell, Colo-Rectal Cancer, 3. Good health, Adenocarcinoma, Papillary, Bioassays and Physiological Analysis, Oncology, Physical Sciences, Colonic Neoplasms, Mechanical Stress, Adenocarcinoma, L-selectin, Anatomy, Antibody, Selectin, Research Article, Colon, General Science & Technology, Research and Analysis Methods, Carcinomas, Fluorescence, 03 medical and health sciences, Formaldehyde, Cell Adhesion, medicine, Humans, Pharmacokinetics, Colorectal Cancer, Pharmacology, Ligand, lcsh:R, Carcinoma, Cancers and Neoplasms, Biology and Life Sciences, Cell Biology, medicine.disease, Molecular biology, Gastrointestinal Tract, 030104 developmental biology, Microscopy, Fluorescence, Selectins, biology.protein, lcsh:Q, Biochemical Analysis, Digestive Diseases, Digestive System, Carcinoma, Signet Ring Cell, Glycoconjugates, Immunostaining |
| الوصف: | A growing body of evidence suggests that L-selectin ligands presented on circulating tumor cells facilitate metastasis by binding L-selectin presented on leukocytes. Commonly used methods for detecting L-selectin ligands on tissues, e.g., immunostaining, are performed under static, no-flow conditions. However, such analysis does not assay for functional L-selectin ligands, specifically those ligands that promote adhesion under shear flow conditions. Recently our lab developed a method, termed dynamic biochemical tissue analysis (DBTA), to detect functional selectin ligands in situ by probing tissues with L-selectin-coated microspheres under hemodynamic flow conditions. In this investigation, DBTA was used to probe human colon tissues for L-selectin ligand activity. The detection of L-selectin ligands using DBTA was highly specific. Furthermore, DBTA reproducibly detected functional L-selectin ligands on diseased, e.g., cancerous or inflamed, tissues but not on noncancerous tissues. In addition, DBTA revealed a heterogeneous distribution of functional L-selectin ligands on colon cancer tissues. Most notably, detection of L-selectin ligands by immunostaining using HECA-452 antibody only partially correlated with functional L-selectin ligands detected by DBTA. In summation, the results of this study demonstrate that DBTA detects functional selectin ligands to provide a unique characterization of pathological tissue. |
| وصف الملف: | application/pdf |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::546741064e9af06d3eb1b7cb582bab9fTest https://doi.org/10.1371/journal.pone.0173747Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....546741064e9af06d3eb1b7cb582bab9f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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