Heat-modified citrus pectin induces apoptosis-like cell death and autophagy in HepG2 and A549 cancer cells
| العنوان: | Heat-modified citrus pectin induces apoptosis-like cell death and autophagy in HepG2 and A549 cancer cells |
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| المؤلفون: | Carine Michiels, Thierry Arnould, Lionel Leclere, Pierre Van Cutsem, Francois Cote, Pierre Cambier, Maude Fransolet |
| المصدر: | PLoS ONE, Vol 10, Iss 3, p e0115831 (2015) Leclere, L, Fransolet, M, Cote, F, Cambier, P, Arnould, T, Van Cutsem, P & Michiels, C 2015, ' Heat-modified citrus pectin induces apoptosis-like cell death and autophagy in HepG2 and A549 cancer cells ', PLoS ONE, vol. 10, no. 3, e0115831 . https://doi.org/10.1371/journal.pone.0115831Test PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Programmed cell death, Hot Temperature, food.ingredient, Pectin, lcsh:Medicine, Apoptosis, Biology, food, Cell Line, Tumor, Autophagy, Humans, Citrus Pectin, lcsh:Science, A549 cell, Multidisciplinary, Adenine, Microfilament Proteins, lcsh:R, Ubiquitination, food and beverages, Hep G2 Cells, Modified Citrus Pectin, Caspase Inhibitors, Cell biology, Biochemistry, Caspases, Cancer cell, Pectins, lcsh:Q, Macrolides, Carrier Proteins, Research Article |
| الوصف: | Cancer is still one of the leading causes of death worldwide, and finding new treatments remains a major challenge. Previous studies showed that modified forms of pectin, a complex polysaccharide present in the primary plant cell wall, possess anticancer properties. Nevertheless, the mechanism of action of modified pectin and the pathways involved are unclear. Here, we show that citrus pectin modified by heat treatment induced cell death in HepG2 and A549 cells. The induced cell death differs from classical apoptosis because no DNA cleavage was observed. In addition, Z-VAD-fmk, a pan-caspase inhibitor, did not influence the observed cell death in HepG2 cells but appeared to be partly protective in A549 cells, indicating that heat-modified citrus pectin might induce caspase-independent cell death. An increase in the abundance of the phosphatidylethanolamine-conjugated Light Chain 3 (LC3) protein and a decrease in p62 protein abundance were observed in both cell types when incubated in the presence of heat-modified citrus pectin. These results indicate the activation of autophagy. To our knowledge, this is the first time that autophagy has been revealed in cells incubated in the presence of a modified form of pectin. This autophagy activation appears to be protective, at least for A549 cells, because its inhibition with 3-methyladenine increased the observed modified pectin-induced cytotoxicity. This study confirms the potential of modified pectin to improve chemotherapeutic cancer treatments. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::740379a5176c3943c22d3d6dad48fd20Test http://europepmc.org/articles/PMC4368604?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....740379a5176c3943c22d3d6dad48fd20 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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