BmTGIF, a Bombyx mori Homolog of Drosophila DmTGIF, Regulates Progression of Spermatogenesis
| العنوان: | BmTGIF, a Bombyx mori Homolog of Drosophila DmTGIF, Regulates Progression of Spermatogenesis |
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| المؤلفون: | Guangli Cao, Renyu Xue, Pengjie Zhang, Chengliang Gong, Jie Sheng |
| المصدر: | PLoS ONE PLoS ONE, Vol 7, Iss 11, p e47861 (2012) |
| بيانات النشر: | Public Library of Science (PLoS), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Anatomy and Physiology, Hemocytes, lcsh:Medicine, Mice, Molecular cell biology, Transcription (biology), Gene Order, Testis, Drosophila Proteins, lcsh:Science, Phylogeny, Multidisciplinary, biology, Drosophila Melanogaster, Agriculture, Spermatid differentiation, Animal Models, Spermatozoa, Recombinant Proteins, Meiosis, Protein Transport, Larva, Insect Proteins, Female, RNA Interference, Drosophila melanogaster, Cell Division, Drosophila Protein, Research Article, Protein Binding, DNA transcription, Molecular Sequence Data, Antibodies, Model Organisms, Bombyx mori, Escherichia coli, Animals, Protein Interaction Domains and Motifs, Amino Acid Sequence, Spermatogenesis, Biology, Transcription factor, Bombyx, Gene Expression Profiling, lcsh:R, Ovary, fungi, Reproductive System, biology.organism_classification, Molecular biology, Alternative Splicing, Gene Expression Regulation, Fertilization, lcsh:Q, Pest Control, Gene expression, Zoology, Entomology, Sequence Alignment, Developmental Biology, Transcription Factors |
| الوصف: | TG-interacting factor (TGIF) in Drosophila consists of two tandemly-repeated genes, achintya (Dmachi) and vismay (Dmvis), which act as transcriptional activators in Drosophila spermatogenesis. In contrast, TGIF in humans is a transcriptional repressor that binds directly to DNA or interacts with corepressors to repress the transcription of target genes. In this study, we investigated the characteristics and functions of BmTGIF, a Bombyx mori homolog of DmTGIF. Like DmTGIF, BmTGIF is predominantly expressed in the testes and ovaries. Four alternatively spliced isoforms could be isolated from testes, and two isoforms from ovaries. Quantitative polymerase chain reaction indicated BmTGIF was abundantly expressed in the testis of 3rd instar larvae, when the testis is almost full of primary spermatocytes. The results of luciferase assays indicated that BmTGIF contains two adjacent acidic domains that activate the transcription of reporter genes. Immunofluorescence assay in BmN cells showed that the BmTGIF protein was located mainly in the nucleus, and paraffin sections of testis showed BmTGIF was grossly expressed in primary spermatocytes and mature sperms. Consistent with the role of DmVis in Drosophila development, BmTGIF significantly affected spermatid differentiation, as indicated by hematoxylin-eosin staining of paraffin sections of testis from BmTGIF-small interfering RNA (siRNA)-injected male silkworms. Co-immunoprecipitation experiments suggested that BmTGIF interacted with BmAly, and that they may recruit other factors to form a complex to regulate the genes required for meiotic divisions and spermatid differentiation. The results of this analysis of BmTGIF will improve our understanding of the mechanism of spermatid differentiation in B. mori, with potential applications for pest control. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be30c2db9c10d5583c8a8aace7209e4eTest https://doi.org/10.1371/journal.pone.0047861Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....be30c2db9c10d5583c8a8aace7209e4e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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