المؤلفون: Omran Allatif, Noemia Mie Orii, Hélène Dutartre, Nicolas Futsch, Augusto César Penalva de Oliveira, Jorge Casseb, Brenda Rocamonde, Renaud Mahieux

المصدر: PLoS Neglected Tropical Diseases, Vol 15, Iss 11, p e0009940 (2021)
PLoS Neglected Tropical Diseases

مصطلحات موضوعية: Male, RNA viruses, Physiology, Cell, RC955-962, Stimulation, NK cells, Pathology and Laboratory Medicine, Monocytes, Cohort Studies, Blood cell, White Blood Cells, Medical Conditions, Animal Cells, immune system diseases, Immune Physiology, hemic and lymphatic diseases, Arctic medicine. Tropical medicine, Tropical spastic paraparesis, Medicine and Health Sciences, Receptor, Immune Response, Human T-lymphotropic virus 1, Innate Immune System, T Cells, virus diseases, Middle Aged, Interleukin-12, Paraparesis, Tropical Spastic, Killer Cells, Natural, Leukemia, Infectious Diseases, medicine.anatomical_structure, Medical Microbiology, Viral Pathogens, Viruses, Cytokines, Female, Cellular Types, Pathogens, Public aspects of medicine, RA1-1270, Brazil, Research Article, Adult, endocrine system, Immune Cells, Inflammatory Diseases, Immunology, Antigen-Presenting Cells, Microbiology, Immune system, Retroviruses, medicine, Humans, Microbial Pathogens, Blood Cells, business.industry, Organisms, Public Health, Environmental and Occupational Health, Biology and Life Sciences, Dendritic Cells, Cell Biology, Htlv-1, Molecular Development, medicine.disease, Immunity, Innate, Immune System, business, Asymptomatic carrier, Developmental Biology

الوصف: The Human T-cell Leukemia Virus-1 (HTLV-1)-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) is a devastating neurodegenerative disease with no effective treatment, which affects an increasing number of people in Brazil. Immune cells from the adaptive compartment are involved in disease manifestation but whether innate cell functions participate in disease occurrence has not been evaluated. In this study, we analyzed innate cell responses at steady state and after blood cell stimulation using an agonist of the toll-like receptor (TLR)7/8-signaling pathway in blood samples from HTLV-1-infected volunteers, including asymptomatic carriers and HAM/TSP patients. We observed a lower response of IFNα+ DCs and monocytes in HAM/TSP compared to asymptomatic carriers, as a potential consequence of corticosteroid treatments. In contrast, a higher frequency of monocytes producing MIP-1α and pDC producing IL-12 was detected in HAM/TSP blood samples, together with higher IFNγ responsiveness of NK cells, suggesting an increased sensitivity to inflammatory response in HAM/TSP patients compared to asymptomatic carriers. This sustained inflammatory responsiveness could be linked or be at the origin of the neuroinflammatory status in HAM/TSP patients. Therefore, the mechanism underlying this dysregulations could shed light onto the origins of HAM/TSP disease.
Author summary The infection by the Human T-cell Leukemia Virus-1 (HTLV-1) is quite frequent in Brazil. Between 1–5% of infected individuals develop a devastating neurodegenerative disease (HAM/TSP) as a result of a sustained inflammation in the central nervous system, with no effective treatment. So far, inflammation has been linked to the deregulated activation of T-cells, but the role of innate cells has not been investigated yet. In this work, we aimed to characterize the responsiveness of innate cells, as this immune population is cornerstone of efficient immune response, but also might participate in disease exacerbation found in chronic infection. Our findings suggest an impaired antiviral response and increased inflammatory responsiveness by dendritic cells and monocytes in HAM/TSP patients compared to asymptomatic carriers. This sustained inflammatory responsiveness upon innate cell activation could participate in the establishment of the HAM/TSP disease.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::886b909f437b8959872deb69e7742a92Test
https://doaj.org/article/db81415417f94efdb20bd8811409b193Test

المؤلفون: James W. Scholey, Igor Jurisica, Ihor Batruch, Anne-Christin Hauschild, Julie A.D. Van, Etienne Sochett, Eleftherios P. Diamandis, Farid H. Mahmud, Yesmino Elia, Sergi Clotet-Freixas, Ana Konvalinka

المصدر: PLoS ONE, Vol 15, Iss 5, p e0233639 (2020)
PLoS ONE

مصطلحات موضوعية: 0301 basic medicine, Proteomics, Male, Proteome, Physiology, 030232 urology & nephrology, Urine, Kidney, Biochemistry, Mass Spectrometry, 0302 clinical medicine, Endocrinology, Medicine and Health Sciences, Hexosaminidase, Child, Extracellular Matrix Proteins, Multidisciplinary, Sulfates, Body Fluids, Chemistry, Proteinuria, medicine.anatomical_structure, Physical Sciences, Medicine, Female, Anatomy, Cellular Structures and Organelles, Research Article, Adult, Adolescent, Endocrine Disorders, Urinary system, Science, Excretion, 03 medical and health sciences, Young Adult, Diabetes mellitus, medicine, Diabetes Mellitus, Humans, KEGG, Type 1 diabetes, business.industry, Chemical Compounds, Biology and Life Sciences, Proteins, Kidneys, Renal System, Cell Biology, medicine.disease, 030104 developmental biology, Diabetes Mellitus, Type 1, Keratan Sulfate, Metabolic Disorders, Immunology, Salts, business, Lysosomes, Physiological Processes

الوصف: Diabetes is the leading cause of end-stage renal disease worldwide. Our understanding of the early kidney response to chronic hyperglycemia remains incomplete. To address this, we first investigated the urinary proteomes of otherwise healthy youths with and without type 1 diabetes and subsequently examined the enriched pathways that might be dysregulated in early disease using systems biology approaches. This cross-sectional study included two separate cohorts for the discovery (N = 30) and internal validation (N = 30) of differentially excreted proteins. Discovery proteomics was performed on a Q Exactive Plus hybrid quadrupole-orbitrap mass spectrometer. We then searched the pathDIP, KEGG, and Reactome databases to identify enriched pathways in early diabetes; the Integrated Interactions Database to retrieve protein-protein interaction data; and the PubMed database to compare fold changes of our signature proteins with those published in similarly designed studies. Proteins were selected for internal validation based on pathway enrichment and availability of commercial enzyme-linked immunosorbent assay kits. Of the 2451 proteins identified, 576 were quantified in all samples from the discovery cohort; 34 comprised the urinary signature for early diabetes after Benjamini-Hochberg adjustment (Q < 0.05). The top pathways associated with this signature included lysosome, glycosaminoglycan degradation, and innate immune system (Q < 0.01). Notably, all enzymes involved in keratan sulfate degradation were significantly elevated in urines from youths with diabetes (|fold change| > 1.6). Increased urinary excretion of monocyte differentiation antigen CD14, hexosaminidase A, and lumican was also observed in the validation cohort (P < 0.05). Twenty-one proteins from our signature have been reported elsewhere as potential mediators of early diabetes. In this study, we identified a urinary proteomic signature for early type 1 diabetes, of which lysosomal enzymes were major constituents. Our findings highlight novel pathways such as keratan sulfate degradation in the early kidney response to hyperglycemia.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8cc005e70c0571f169ae7df37a15ee84Test
https://doaj.org/article/dce7c9a00e8e4ff7be56ce4cb65ccd87Test

المؤلفون: Virginie Neirinckx, Sabine Wislet, Jacques Foguenne, Anil Sharma, Gulistan Agirman, Francois Lallemend, Cécile Coste, André Gothot, Bernard Rogister

المصدر: PLoS ONE, Vol 16, Iss 9, p e0256484 (2021)
PLoS ONE
PLoS ONE, Vol 12, Iss 7, p e0177962 (2017)

مصطلحات موضوعية: 0301 basic medicine, Embryology, Cellular differentiation, lcsh:Medicine, Gene Expression, Adipose tissue, Chick Embryo, Biochemistry, Nestin, Neural Stem Cells, Animal Cells, Medicine and Health Sciences, lcsh:Science, Neurons, Transcription Factor Brn-3A, Multidisciplinary, Stem Cells, Nuclear Proteins, RNA-Binding Proteins, Neural crest, SOX9 Transcription Factor, Cell Differentiation, Dermis, Cell biology, medicine.anatomical_structure, Adipose Tissue, Neural Crest, Melanocytes, Medicine, Female, Cellular Types, Anatomy, Neuronal Differentiation, Research Article, Adult, Stromal cell, Microinjections, Science, Nerve Tissue Proteins, Bone Marrow Cells, Receptors, Nerve Growth Factor, Biology, Mesenchymal Stem Cell Transplantation, 03 medical and health sciences, medicine, Animals, Humans, Adults, lcsh:R, Twist-Related Protein 1, Mesenchymal stem cell, Embryos, Correction, Biology and Life Sciences, Proteins, Mesenchymal Stem Cells, Cell Biology, Cytoskeletal Proteins, 030104 developmental biology, Biological Tissue, Age Groups, People and Places, lcsh:Q, Population Groupings, Schwann Cells, Snail Family Transcription Factors, Bone marrow, Biomarkers, Developmental Biology

الوصف: Adult neural crest stem-derived cells (NCSC) are of extraordinary high plasticity and promising candidates for use in regenerative medicine. Several locations such as skin, adipose tissue, dental pulp or bone marrow have been described in rodent, as sources of NCSC. However, very little information is available concerning their correspondence in human tissues, and more precisely for human bone marrow. The main objective of this study was therefore to characterize NCSC from adult human bone marrow. In this purpose, we compared human bone marrow stromal cells to human adipose tissue and dermis, already described for containing NCSC. We performed comparative analyses in terms of gene and protein expression as well as functional characterizations. It appeared that human bone marrow, similarly to adipose tissue and dermis, contains NESTIN+ / SOX9+ / TWIST+ / SLUG+ / P75NTR+ / BRN3A+/ MSI1+/ SNAIL1+ cells and were able to differentiate into melanocytes, Schwann cells and neurons. Moreover, when injected into chicken embryos, all those cells were able to migrate and follow endogenous neural crest migration pathways. Altogether, the phenotypic characterization and migration abilities strongly suggest the presence of neural crest-derived cells in human adult bone marrow.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffd6a6fb37955343775dd52e3439038eTest
https://doaj.org/article/39552e5a28684c0ba5897ce6f678e54fTest

المؤلفون: Ebenezer Addo Ofori, Maria Belmonte, Bjoern Peters, Martha Sedegah, Eileen Villasante, Harini Ganeshan, Omarine Nfor Nlinwe, Kwadwo Akyea-Mensah, Eric Kyei-Baafour, Kwadwo A. Kusi

المصدر: PLoS ONE, Vol 16, Iss 9, p e0257219 (2021)
PLoS ONE

مصطلحات موضوعية: Male, Protozoan Proteins, Epitopes, T-Lymphocyte, CD8-Positive T-Lymphocytes, Biochemistry, Epitope, White Blood Cells, Medical Conditions, Animal Cells, Medicine and Health Sciences, Cytotoxic T cell, Public and Occupational Health, Malaria, Falciparum, Amino Acids, Enzyme-Linked Immunoassays, Immune Response, Protozoans, Multidisciplinary, Organic Compounds, T Cells, ELISPOT, Malarial Parasites, Eukaryota, Middle Aged, Vaccination and Immunization, Chemistry, medicine.anatomical_structure, Physical Sciences, Medicine, Female, Cellular Types, Research Article, Adult, Immune Cells, T cell, Science, Plasmodium falciparum, Immunology, Antigens, Protozoan, Human leukocyte antigen, Biology, Research and Analysis Methods, Young Adult, Antigen, Malaria Vaccines, Vaccine Development, Parasitic Diseases, medicine, Humans, Apical membrane antigen 1, Immunoassays, Alleles, Blood Cells, Organic Chemistry, T-cell receptor, Organisms, Chemical Compounds, Biology and Life Sciences, Proteins, Cell Biology, Tropical Diseases, Parasitic Protozoans, Malaria, Amino Acid Substitution, Immunologic Techniques, Preventive Medicine, Peptides

الوصف: Antigen polymorphisms in essential malarial antigens are a key challenge to the design and development of broadly effective malaria vaccines. The effect of polymorphisms on antibody responses is fairly well studied while much fewer studies have assessed this for T cell responses. This study investigated the effect of allelic polymorphisms in the malarial antigen apical membrane antigen 1 (AMA1) onex vivoT cell-specific IFN-γ responses in subjects with lifelong exposure to malaria. Human leukocyte antigen (HLA) class I-restricted peptides from the 3D7 clone AMA1 were bioinformatically predicted and those with variant amino acid positions used to select corresponding allelic sequences from the 7G8, FVO, FC27 and tm284 parasite strains. A total of 91 AMA1 9-10mer peptides from the five parasite strains were identified, synthesized, grouped into 42 allele sets and used to stimulate PBMCs from seven HLA class 1-typed subjects in IFN-γ ELISpot assays. PBMCs from four of the seven subjects (57%) made positive responses to 18 peptides within 12 allele sets. Fifty percent of the 18 positive peptides were from the 3D7 parasite variant. Amino acid substitutions that were associated with IFN-γ response abrogation were more frequently found at positions 1 and 6 of the tested peptides, but substitutions did not show a clear pattern of association with response abrogation. Thus, while we show some evidence of polymorphisms affecting T cell response induction, other factors including TCR recognition of HLA-peptide complexes may also be at play.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::feae6d60d5975971023cc32f97d35aaeTest
https://doaj.org/article/584a3bd4a35d4faaa695c701e2bdbb7eTest

المؤلفون: Anthony M. Magliocco, Robert M. Wenham, Susan M McCarthy, Sachin M. Apte, Jennifer B. Permuth, Dung-Tsa Chen, Ardeshir Hakam, Johnathan M. Lancaster, Douglas C. Marchion, Daryoush Saeed-Vafa, Alexis S. Lopez, Brett M. Reid

المصدر: PLoS ONE, Vol 16, Iss 11, p e0256615 (2021)
PLoS ONE

مصطلحات موضوعية: endocrine system diseases, Caveolin 1, Cancer Treatment, Epithelium, Metastasis, Breast Tumors, Medicine and Health Sciences, Aged, 80 and over, Ovarian Neoplasms, Multidisciplinary, medicine.diagnostic_test, Prostate Cancer, Prostate Diseases, Obstetrics and Gynecology, Middle Aged, Immunohistochemistry, Ovarian Cancer, Gene Expression Regulation, Neoplastic, Survival Rate, Serous fluid, medicine.anatomical_structure, Oncology, cardiovascular system, Biomarker (medicine), Medicine, Female, Anatomy, Research Article, Adult, Stromal cell, Urology, Science, Down-Regulation, Immunofluorescence, Malignant Tumors, Stroma, Breast Cancer, medicine, Biomarkers, Tumor, Humans, Aged, Neoplasm Staging, business.industry, Ovary, Gynecologic Cancers, Cancers and Neoplasms, Biology and Life Sciences, medicine.disease, Cystadenocarcinoma, Serous, Genitourinary Tract Tumors, Biological Tissue, Tissue Array Analysis, Cancer research, Women's Health, Stromal Cells, business, Gynecological Tumors

الوصف: Loss of stromal caveolin-1 (Cav-1) is a biomarker of a cancer-associated fibroblast (CAF) phenotype and is related to progression, metastasis, and poor outcomes in several cancers. The objective of this study was to evaluate the clinical significance of Cav-1 expression in invasive epithelial ovarian cancer (OvCa). Epithelial and stromal Cav-1 expression were quantified in serous OvCa and benign ovarian tissue in two, independent cohorts–one quantified expression using immunohistochemistry (IHC) and the other using multiplex immunofluorescence (IF) with digital image analysis designed to target CAF-specific expression. Cav-1 expression was significantly downregulated in OvCa stroma compared to non-neoplastic stroma using both the IHC (p = 0.002) and IF (p = 1.8x10-13) assays. OvCa stroma showed Cav-1 downregulation compared to tumor epithelium with IHC (p = 1.2x10-24). Conversely, Cav-1 expression was higher in OvCa stroma compared to tumor epithelium with IF (p = 0.002). There was moderate correlation between IHC and IF methods for stromal Cav-1 expression (r2 = 0.69, p = 0.006) whereas there was no correlation for epithelial expression (r2 = 0.006, p = 0.98). Irrespective of the staining assay, neither response to therapy or overall survival correlated with the expression level of Cav-1 in the stroma or tumor epithelium. Our findings demonstrate a loss of stromal Cav-1 expression in ovarian serous carcinomas. Studies are needed to replicate these findings and explore therapeutic implications, particularly for immunotherapy response.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ccab72a6d0b4a13c7fdbbfce8e00d8eTest
https://doaj.org/article/5ca8a7d61e0f4af186e6fa6b2b9a9a6fTest

المؤلفون: Mohammad Javan, Seyed Massood Nabavi, Forough Foolad, Fariba Khodagholi

المصدر: PLoS ONE, Vol 15, Iss 3, p e0230691 (2020)
PLoS ONE

مصطلحات موضوعية: 0301 basic medicine, Male, Central Nervous System, Physiology, Pathology and Laboratory Medicine, Gastroenterology, Severity of Illness Index, Biochemistry, Nervous System, 0302 clinical medicine, Sirtuin 1, Infectious Diseases of the Nervous System, Sirtuin 3, Blood plasma, Pyruvic Acid, Medicine and Health Sciences, Public and Occupational Health, Energy-Producing Organelles, Cognitive Impairment, Multidisciplinary, Cognitive Neurology, Neuromyelitis Optica, Cytochromes c, Middle Aged, Myelitis, Ketones, Mitochondria, Body Fluids, Chemistry, medicine.anatomical_structure, Infectious Diseases, Blood, Neurology, Physical Sciences, Medicine, Female, Cellular Structures and Organelles, Anatomy, Research Article, Adult, Pyruvate, medicine.medical_specialty, SIRT3, Cognitive Neuroscience, Disabilities, Science, Central nervous system, Bioenergetics, Peripheral blood mononuclear cell, Blood Plasma, Electron Transport Complex IV, 03 medical and health sciences, Signs and Symptoms, Diagnostic Medicine, Internal medicine, Severity of illness, medicine, Humans, Lactic Acid, Neuromyelitis optica, Electron Transport Complex I, business.industry, Case-control study, Chemical Compounds, Biology and Life Sciences, Cell Biology, medicine.disease, 030104 developmental biology, Case-Control Studies, Leukocytes, Mononuclear, Lesions, Cognitive Science, business, Cognition Disorders, Acids, 030217 neurology & neurosurgery, Neuroscience

الوصف: BackgroundNeuromyelitis Optica (NMO) is an inflammatory demyelinating disease that mainly affects optic nerves and spinal cord. Besides, loss of motor and cognitive function has been reported as important symptoms of disease.ObjectiveHere we investigated the mitochondrial dysfunction and metabolic alterations in NMO patients and evaluate their correlation with disease progress, disability and cognitive impairment.MethodsThe individuals (12 controls and 12 NMO) were assessed for disease severity by expanded disease status scale (EDSS), cognitive function via symbol digit modalities test (SDMT) and fine motor disability by 9-hole peg test (9-HPT). We have measured Sirtuin 1 (SIRT1), SIRT3, mitochondrial complex I, complex IV, aconitase and α-ketoglutarate dehydrogenase (α-KGD) activity in peripheral blood mononuclear cells (PBMCs). Furthermore, SIRT1, pyruvate, lactate and cytochrome c (Cyt c) were determined in plasma.ResultsOur results exhibited increased 9-HPT time in NMO patients. 9-HPT results correlated with EDSS; and SDMT negatively correlated with disease duration and number of attacks in patients. Investigation of PBMCs of NMO patients exhibited a decrease of mitochondrial complex I and IV activity that was significant for complex IV. Besides, complex I activity was negatively correlated with 9-HPT time in NMO group. In the plasma samples, a correlation between pyruvate to lactate ratio and EDSS in NMO patients was found and a negative correlation between Cyt c concentration and SDMT was detected.ConclusionOur data support the hypothesis that mitochondrial dysfunction occurred in the CNS and the peripheral blood may contribute to disease progress, disability level and the cognitive impairment in NMO patients.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82d52e6a5d7d0782a088e658a3c8f509Test
https://doaj.org/article/d28ef3ea08da4e81a93811db56e98da1Test

المؤلفون: Jiaying An, Weiwei Zhao, Shihui Yu, Rongchang Chen, Xiaoxian Zhang, Jie Liu, Ou Xiaohua, Shiyue Li, Nanshan Zhong, Sun Mingming, Hu Changming, Deng Junhao, Zhen Zhao, Feifei Liu, Weili Gu, Jiaxing Xie, Qingling Zhang

المصدر: PLoS ONE, Vol 15, Iss 1, p e0228066 (2020)
PLoS ONE

مصطلحات موضوعية: Oncology, Adult, medicine.medical_specialty, China, DNA Copy Number Variations, Science, Polymorphism, Single Nucleotide, Tuberous Sclerosis Complex 1 Protein, Internal medicine, Tuberous Sclerosis Complex 2 Protein, medicine, Humans, Lymphangioleiomyomatosis, Multidisciplinary, business.industry, Correction, medicine.disease, medicine.anatomical_structure, Mutation (genetic algorithm), Mutation, Medicine, Female, TSC1, TSC2, business

الوصف: The aim of our study was to elucidate the landscapes of genetic alterations of TSC1 and TSC2 as well as other possible non-TSC1/2 in Lymphangioleiomyomatosis (LAM) patients. Sixty-one Chinese LAM patients' clinical information was collected. Tumor biopsies and matched leukocytes from these patients were retrospectively analyzed by next generation sequencing (NGS), chromosomal microarray analysis (CMA), and multiplex ligation-dependent probe amplification (MLPA). Eighty-six TSC1/2 variants were identified in 46 of the 61 LAM patients (75.4%) in which TSC2 and TSC1 variants were 88.37% and 11.63% respectively. The 86 variants are composed of (i) 52 single nucleotide variants (SNVs) (including 30 novel variants), (ii) 23 indels (including 21deletions, and 2 insertions), (iii) a germline duplication of exon 31-42 of TSC2, (iv) a 2.68 Mb somatic duplication containing TSC2, and (v) 9 regions with copy-neutral loss of heterogeneity (CN-LOHs) present only in the LAM patients with single TSC1/2 mutations. Sixty-one non-TSC1/2 variants in 31 genes were identified in 37 LAM patients. Combined applications of different techniques are necessary to achieve maximal detection rate of TSC1/2 variants in LAM patients. Thirty novel TSC1/2 variants expands the spectrum of TSC1/2 in LAM patients. Identification of 61 non-TSC1/2 variants suggests that alternative genes might have contributed to the initiation and progression of LAM.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec385ef9da391b5b4799469a357c246cTest
https://doaj.org/article/5526881952fd447194ebe105bd4c1b00Test

المؤلفون: Etienne Larger, Marc Diedisheim, Roberto Mallone, Fidéline Bonnet-Serrano

المصدر: PLoS ONE, Vol 13, Iss 1, p e0191528 (2018)
PLoS ONE

مصطلحات موضوعية: 0301 basic medicine, Male, Physiology, medicine.medical_treatment, Peptide Hormones, lcsh:Medicine, Biochemistry, 0302 clinical medicine, Endocrinology, Fibrosis, Insulin-Secreting Cells, Medicine and Health Sciences, Child, lcsh:Science, Multidisciplinary, Organ Size, Middle Aged, Pancreas, Exocrine, Tissue Donors, medicine.anatomical_structure, Adipose Tissue, Physiological Parameters, Female, Anatomy, Pancreas, Research Article, Adult, medicine.medical_specialty, Adolescent, Endocrine Disorders, Imaging Techniques, Urology, 030209 endocrinology & metabolism, Endocrine System, Research and Analysis Methods, Glucagon, 03 medical and health sciences, Young Adult, Exocrine Glands, Diabetes mellitus, Image Interpretation, Computer-Assisted, medicine, Diabetes Mellitus, Humans, Total Tissue, Secretion, Type 1 diabetes, business.industry, Insulin, Morphometry, Body Weight, lcsh:R, Case-control study, Biology and Life Sciences, medicine.disease, Hormones, Hypoglycemia, 030104 developmental biology, Diabetes Mellitus, Type 1, Glucagon-Secreting Cells, Case-Control Studies, Metabolic Disorders, lcsh:Q, business, Physiological Processes, Developmental Biology

الوصف: Background and aims Abnormal glucagon secretion and functional alterations of the exocrine pancreas have been described in patients with type 1 diabetes (T1D), but their respective anatomical substrata have seldom been investigated. Our aim was to develop an automated morphometric analysis process to characterize the anatomy of α-cell and exocrine pancreas in patients with T1D, using the publicly available slides of the Network for Pancreatic Organ Donors (nPOD). Materials and methods The ratio of β- and α-cell area to total tissue area were quantified in 75 patients with T1D (thereafter patients) and 66 control subjects (thereafter controls), on 2 insulin-stained and 4 glucagon-stained slides from both the head and the tail of the pancreas. The β- and α-cell masses were calculated in the 66 patients and the 50 controls for which the pancreas weight was available. Non-exocrine-non-endocrine tissue area (i.e. non-acinar, non-insular tissue) to total tissue area ratio was evaluated on both insulin- and glucagon-stained slides. Results were expressed as mean ±SD. Results An automated quantification method was set up using the R software and was validated by quantification of β-cell mass, a well characterized parameter. β-cell mass was 29.6±112 mg in patients and 628 ±717 mg in controls (p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73013132847536146c4c2810731e2350Test
http://europepmc.org/articles/PMC5774815?pdf=renderTest

المؤلفون: A.O. Abdelkareem, Fahad T. Alotaibi, Natasha L. Orr, Heather Noga, Bo Peng, Christian Klausen, Anna F. Lee, Mohamed A. Bedaiwy, Paul J. Yong

المصدر: PLoS ONE, Vol 14, Iss 7, p e0219064 (2019)
PLoS ONE

مصطلحات موضوعية: 0301 basic medicine, Vaginal Diseases, Endometriosis, Artificial Gene Amplification and Extension, Peritoneal Diseases, Endometrium, Biochemistry, Polymerase Chain Reaction, Epithelium, chemistry.chemical_compound, 0302 clinical medicine, Animal Cells, Medicine and Health Sciences, Small interfering RNAs, media_common, Uterine Diseases, Staining, 030219 obstetrics & reproductive medicine, Multidisciplinary, Obstetrics and Gynecology, Cell Staining, Middle Aged, Immunohistochemistry, 3. Good health, Nucleic acids, medicine.anatomical_structure, Plasminogen activator inhibitor-1, Medicine, Female, Anatomy, Cellular Types, medicine.symptom, Research Article, Adult, medicine.medical_specialty, media_common.quotation_subject, Science, Urology, Research and Analysis Methods, Young Adult, 03 medical and health sciences, Dysmenorrhea, Uterine cancer, Plasminogen Activator Inhibitor 1, Genetics, medicine, Humans, Non-coding RNA, Molecular Biology Techniques, Immunohistochemistry Techniques, Molecular Biology, Menstrual cycle, Biology and life sciences, business.industry, Pelvic pain, Uterus, Reproductive System, Dysmenorrhoea, Epithelial Cells, Cell Biology, medicine.disease, Gene regulation, Histochemistry and Cytochemistry Techniques, Rectal Diseases, Biological Tissue, 030104 developmental biology, chemistry, Specimen Preparation and Treatment, Immunologic Techniques, Women's Health, RNA, Gene expression, Stromal Cells, business, Plasminogen activator, Menstrual Abnormalities

الوصف: PurposeDeep infiltrating endometriosis (DIE) is defined as an endometriotic lesion penetrating to a depth of >5 mm and is associated with pelvic pain, but the underlying mechanisms are unclear. Our objective is to investigate whether plasminogen activator inhibitor-1 expression (PAI-1) in endometriotic tissues is increased in women with DIE.MethodsIn this blinded in vitro study, immunohistochemistry and Histoscore were used to examine the expression of PAI-1 in glandular epithelium (GECs) and stroma (SCs) in a total of 62 women: deep infiltrating uterosacral/rectovaginal endometriosis (DIE; n = 13), ovarian endometrioma (OMA; n = 14), superficial peritoneal uterosacral/cul-de-sac endometriosis (SUP; n = 23), uterine (eutopic) endometrium from women with endometriosis (UE; n = 6), and non-endometriosis eutopic endometrium (UC; n = 6). The following patient characteristics were also collected: age, American Fertility Society stage, hormonal suppression, phase of menstrual cycle, dysmenorrhea score and deep dyspareunia score.ResultsPAI-1 expression in GECs and SCs of the DIE group was significantly higher than that of SUP group (p = 0.01, p = 0.01, respectively) and UE group (p = 0.03, p = 0.04, respectively). Interestingly, increased PAI-1 expression in GECs and SCs was also significantly correlated with increased dysmenorrhea (r = 0.38, p = 0.01; r = 0.34, p = 0.02, respectively).ConclusionsWe found higher expression of PAI-1 in DIE, and an association between PAI-1 and worse dysmenorrhea.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2105f53250278bcc07b0ddd776e772f4Test
https://doaj.org/article/877102c416744af291f301344f1372f9Test

المؤلفون: Ali Erginay, Ramin Tadayoni, Aude Couturier, Alain Gaudric, Bénédicte Dupas, Carlo Lavia

المصدر: PLoS ONE, Vol 14, Iss 7, p e0219164 (2019)
PLoS ONE

مصطلحات موضوعية: 0301 basic medicine, Male, Diagnostic Radiology, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Medicine and Health Sciences, Fluorescein Angiography, Tomography, Multidisciplinary, medicine.diagnostic_test, Radiology and Imaging, Inner limiting membrane, Diabetic retinopathy, Middle Aged, Fluorescein angiography, Arterioles, medicine.anatomical_structure, Retinal Disorders, Medicine, Female, Anatomy, Cellular Structures and Organelles, Tomography, Optical Coherence, Research Article, Adult, medicine.medical_specialty, Imaging Techniques, Endocrine Disorders, Science, Outer plexiform layer, Research and Analysis Methods, Retina, 03 medical and health sciences, Young Adult, Vessel density, Nuclear Membrane, Ocular System, Diagnostic Medicine, Ophthalmology, medicine, Diabetes Mellitus, Humans, Retinopathy, Macular edema, Retrospective Studies, Cell Nucleus, Diabetic Retinopathy, Retinal Vessels, Biology and Life Sciences, Retinal, Cell Biology, medicine.disease, Inner plexiform layer, Capillaries, 030104 developmental biology, Diabetes Mellitus, Type 1, chemistry, Case-Control Studies, Metabolic Disorders, 030221 ophthalmology & optometry, Cardiovascular Anatomy, Eyes, Blood Vessels, sense organs, Head

الوصف: PurposeTo explore the relationships between vessel density (VD) in the retinal vascular plexuses with the thickness and structural changes of their corresponding retinal layers in patients with diabetic retinopathy (DR).MethodsRetrospective analysis of 17 eyes of 17 Type 1 diabetes (T1D) patients with severe non-proliferative or proliferative DR and no current or past macular edema. Seventeen age- and sex-matched healthy subjects were included as controls. Using optical coherence tomography (OCT) and OCT-angiography (OCTA), VD was measured in the superficial vascular plexus (SVP) and deep vascular complex (DVC) that includes the intermediate (ICP) and deep capillary plexuses (DCP), and compared to the retinal thickness (RT) of the inner (from the inner limiting membrane to the inner plexiform layer) and intermediate (inner nuclear and outer plexiform layer) retinal layers. The correlation between the inner and intermediate RT and the VD of the corresponding vascular networks (SVP and DVC, respectively) was assessed. All OCT and OCTA examinations were performed using the RTVue XR Avanti (Optovue, Fremont, CA).ResultsThe inner RT and VD in all plexuses were significantly reduced in T1D patients compared to healthy subjects. The capillary drop-out patterns were polygonal and well-defined in the SVP while the ICP and DCP showed a more diffuse capillary rarefaction and a VD that varied in the same proportion. The inner RT significantly correlated with VD in the SVP (r = 0.71 in healthy subjects and r = 0.62 in T1D patients, p ConclusionsIn T1D subjects, OCTA allowed observing different capillary drop-out patterns in the SVP and in the ICP-DCP, with different structural changes in the corresponding retinal layers, suggesting that they should be considered as distinct anatomical and functional entities.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4ec420739028296e366ad8b6fecc2d4Test
https://doaj.org/article/d430017de5874f2a9405e500b2c3ccb5Test