Activation of AMP-Activated Protein Kinase by Adenine Alleviates TNF-Alpha-Induced Inflammation in Human Umbilical Vein Endothelial Cells
| العنوان: | Activation of AMP-Activated Protein Kinase by Adenine Alleviates TNF-Alpha-Induced Inflammation in Human Umbilical Vein Endothelial Cells |
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| المؤلفون: | Guang-Huar Young, Jiun-Tsai Lin, Yao-Jen Liang, Shao-Hsuan Kao, Jing-Yi Nong, Po-Ku Chen, Chin-Chen Chen, Hyun-Hwa Jang, Han-Min Chen, Yi-Fang Cheng, Cheng-Yi Kuo |
| المصدر: | PLoS ONE, Vol 10, Iss 11, p e0142283 (2015) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adenine Phosphoribosyltransferase, Adenine phosphoribosyltransferase, Gene Expression, lcsh:Medicine, AMP-Activated Protein Kinases, Monocytes, Umbilical vein, Small hairpin RNA, AMP-activated protein kinase, Cell Adhesion, Human Umbilical Vein Endothelial Cells, Humans, Phosphorylation, RNA, Small Interfering, lcsh:Science, Protein kinase A, Cells, Cultured, Inflammation, Multidisciplinary, biology, Tumor Necrosis Factor-alpha, Activator (genetics), Adenine, lcsh:R, NF-kappa B, AMPK, Ribonucleotides, Aminoimidazole Carboxamide, Molecular biology, Enzyme Activation, Protein Transport, biology.protein, lcsh:Q, Research Article |
| الوصف: | The AMP-activated protein kinase (AMPK) signaling system plays a key role in cellular stress by repressing the inflammatory responses induced by the nuclear factor-kappa B (NF-κB) system. Previous studies suggest that the anti-inflammatory role of AMPK involves activation by adenine, but the mechanism that allows adenine to produce these effects has not yet been elucidated. In human umbilical vein endothelial cells (HUVECs), adenine was observed to induce the phosphorylation of AMPK in both a time- and dose-dependent manner as well as its downstream target acetyl Co-A carboxylase (ACC). Adenine also attenuated NF-κB targeting of gene expression in a dose-dependent manner and decreased monocyte adhesion to HUVECs following tumor necrosis factor (TNF-α) treatment. The short hairpin RNA (shRNA) against AMPK α1 in HUVECs attenuated the adenine-induced inhibition of NF-κB activation in response to TNF-α, thereby suggesting that the anti-inflammatory role of adenine is mediated by AMPK. Following the knockdown of adenosyl phosphoribosyl transferase (APRT) in HUVECs, adenine supplementation failed to induce the phosphorylation of AMPK and ACC. Similarly, the expression of a shRNA against APRT nullified the anti-inflammatory effects of adenine in HUVECs. These results suggested that the role of adenine as an AMPK activator is related to catabolism by APRT, which increases the cellular AMP levels to activate AMPK. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1debb301f2585083b34a3d23a3f20f8Test https://doi.org/10.1371/journal.pone.0142283Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f1debb301f2585083b34a3d23a3f20f8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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