O:2-CRM(197) conjugates against Salmonella Paratyphi A
| العنوان: | O:2-CRM(197) conjugates against Salmonella Paratyphi A |
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| المؤلفون: | Luisa Lanzilao, Donata Medaglini, Francesca Micoli, Laura B. Martin, Allan Saul, Massimiliano Gavini, Simona Rondini |
| المصدر: | PLoS ONE, Vol 7, Iss 11, p e47039 (2012) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Bacterial Diseases, Serotype, Glycobiology, lcsh:Medicine, Biochemistry, Analytical Chemistry, Mice, 0302 clinical medicine, Conjugate vaccines, Salmonella vaccines, Salmonella Thypi, Salmonella, lcsh:Science, Chromatography, Vaccines, 0303 health sciences, Multidisciplinary, Vaccination, Chemical Reactions, Salmonella paratyphi A, O Antigens, 3. Good health, Chemistry, Infectious Diseases, Carbohydrate Sequence, Salmonella enterica serovar Typhi, Medicine, Female, Antibody, Research Article, Neglected Tropical Diseases, Molecular Sequence Data, 030231 tropical medicine, Carbohydrates, Biology, Bivalent (genetics), Microbiology, 03 medical and health sciences, Chemical Analysis, Bacterial Proteins, Antigen, Polysaccharides, Vaccine Development, Paratyphoid Fever, Animals, Humans, Glycoproteins, 030304 developmental biology, Vaccines, Conjugate, Typhoid-Paratyphoid Vaccines, lcsh:R, Immunity, Virology, Protective antigen, biology.protein, Clinical Immunology, lcsh:Q, Conjugate |
| الوصف: | Enteric fevers remain a common and serious disease, affecting mainly children and adolescents in developing countries. Salmonella enterica serovar Typhi was believed to cause most enteric fever episodes, but several recent reports have shown an increasing incidence of S. Paratyphi A, encouraging the development of a bivalent vaccine to protect against both serovars, especially considering that at present there is no vaccine against S. Paratyphi A. The O-specific polysaccharide (O:2) of S. Paratyphi A is a protective antigen and clinical data have previously demonstrated the potential of using O:2 conjugate vaccines. Here we describe a new conjugation chemistry to link O:2 and the carrier protein CRM(197), using the terminus 3-deoxy-D-manno-octulosonic acid (KDO), thus leaving the O:2 chain unmodified. The new conjugates were tested in mice and compared with other O:2-antigen conjugates, synthesized adopting previously described methods that use CRM(197) as carrier protein. The newly developed conjugation chemistry yielded immunogenic conjugates with strong serum bactericidal activity against S. Paratyphi A. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d1e5991786afe518ecf8ce1604af028Test http://europepmc.org/articles/PMC3492368?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4d1e5991786afe518ecf8ce1604af028 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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