C/EBPalpha and C/EBPbeta are required for Sebocyte differentiation and stratified squamous differentiation in adult mouse skin
| العنوان: | C/EBPalpha and C/EBPbeta are required for Sebocyte differentiation and stratified squamous differentiation in adult mouse skin |
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| المؤلفون: | Keith E. Linder, Songyun Zhu, Robert C. Smart, Rakesh Ranjan, John S. House |
| المصدر: | PLoS ONE, Vol 5, Iss 3, p e9837 (2010) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Keratinocytes, medicine.medical_specialty, Genotype, Squamous Differentiation, Cellular differentiation, Immunoblotting, Preputial gland, Cell Biology/Cell Growth and Division, Meibomian gland, lcsh:Medicine, Dermatology, Dermatology/Dermatologic Pathology, Biology, 03 medical and health sciences, Basal (phylogenetics), Mice, Sebaceous Glands, 0302 clinical medicine, Internal medicine, medicine, CCAAT-Enhancer-Binding Protein-alpha, Animals, lcsh:Science, Melanocortin 5 receptor, Cell Biology/Gene Expression, 030304 developmental biology, Cell Proliferation, Skin, 0303 health sciences, Multidisciplinary, Epidermis (botany), integumentary system, CCAAT-Enhancer-Binding Protein-beta, Cell Cycle, Homozygote, digestive, oral, and skin physiology, lcsh:R, Cell Differentiation, Epithelial Cells, Molecular biology, Immunohistochemistry, medicine.anatomical_structure, Endocrinology, Gene Expression Regulation, 030220 oncology & carcinogenesis, lcsh:Q, biological phenomena, cell phenomena, and immunity, Keratinocyte, Research Article |
| الوصف: | C/EBPalpha and C/EBPbeta are bZIP transcription factors that are highly expressed in the interfollicular epidermis and sebaceous glands of skin and yet germ line deletion of either family member alone has only mild or no effect on keratinocyte biology and their role in sebocyte biology has never been examined. To address possible functional redundancies and reveal functional roles of C/EBPalpha and C/EBPbeta in postnatal skin, mouse models were developed in which either family member could be acutely ablated alone or together in the epidermis and sebaceous glands of adult mice. Acute removal of either C/EBPalpha or C/EBPbeta alone in adult mouse skin revealed modest to no discernable changes in epidermis or sebaceous glands. In contrast, co-ablation of C/EBPalpha and C/EBPbeta in postnatal epidermis resulted in disruption of stratified squamous differentiation characterized by hyperproliferation of basal and suprabasal keratinocytes and a defective basal to spinous keratinocyte transition involving an expanded basal compartment and a diminished and delayed spinous compartment. Acute co-ablation of C/EBPalpha and C/EBPbeta in sebaceous glands resulted in severe morphological defects, and sebocyte differentiation was blocked as determined by lack of sebum production and reduced expression of stearoyl-CoA desaturase (SCD3) and melanocortin 5 receptor (MC5R), two markers of terminal sebocyte differentiation. Specialized sebocytes of Meibomian glands and preputial glands were also affected. Our results indicate that in adult mouse skin, C/EBPalpha and C/EBPbeta are critically involved in regulating sebocyte differentiation and epidermal homeostasis involving the basal to spinous keratinocyte transition and basal cell cycle withdrawal. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0988432ccded46580ba85c9a1a81d9e7Test http://europepmc.org/articles/PMC2843749?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0988432ccded46580ba85c9a1a81d9e7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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