التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of Peregrinus maidis transformer-2 as a target for CRISPR-based control. |
| المؤلفون: |
Wang, Yu-Hui1 (AUTHOR), Rivera, Dina Espinoza1 (AUTHOR), Klobasa, William1 (AUTHOR), Lorenzen, Marcé D.1 (AUTHOR) marce_lorenzen@ncsu.edu |
| المصدر: |
PLoS ONE. 4/18/2024, Vol. 19 Issue 4, p1-13. 13p. |
| مصطلحات موضوعية: |
*CRISPRS, *RNA interference, *SMALL interfering RNA, *GENOME editing, *NILAPARVATA lugens |
| مستخلص: |
The corn planthopper, Peregrinus maidis, is an economically important pest of corn and sorghum. Here we report the initial steps towards developing a CRISPR-based control method, precision guided sterile insect technique (pgSIT), for this hemipteran pest. Specifically, we evaluated the potential of transformer-2 (tra-2) as a target for sterilizing insects. First, we identified tra-2 transcripts within our P. maidis transcriptome database and performed RNA interference (RNAi) to confirm functional conservation. RNAi-mediated knockdown of Pmtra-2 in nymphs transformed females into pseudomales with deformed ovipositors resembling male claspers. While males showed no overt difference in appearance, they were indeed sterile. Importantly, the results were similar to those observed in another planthopper, Nilaparvata lugens. We also used CRISPR/Cas9 genome editing to assess the impact of tra-2 knockout in injectees. CRISPR-mediated knockout of Pmtra-2 had lethal effects on embryos, and hence not many injectees reached adulthood. However, mosaic knockout of Pmtra-2 did impact female and male fertility, which supports the use of tra-2 as a target for pgSIT in this hemipteran species. [ABSTRACT FROM AUTHOR] |
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