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1دورية أكاديمية
المؤلفون: De Angelis G., Menchinelli G., Torelli R., De Carolis E., Sanguinetti M., Posteraro B.
المساهمون: De Angelis, Giulia, Menchinelli, Giulia, Torelli, Riccardo, De Carolis, Elena, Posteraro, P., Sanguinetti, Maurizio, Posteraro, Brunella
مصطلحات موضوعية: Culture media, Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
الوصف: The aim of this study was to compare the Candida bromcresol green (BCG) medium with the chromogenic (CHROM) Brilliance Candida agar and Sabouraud dextrose agar (SDA) media in regard to their capability of detecting Candida isolates from mono- or dual-species cultures. We prepared Candida isolates' suspensions to obtain mono-species (n = 18) or dual-species (n = 153) culture plates per each medium, and three readers independently observed 513 plates at 24-h, 48-h and 72-h incubation time. We scored reading results as correct, over or under detection compared to the expected species number(s). BCG showed significantly higher correct-detection and lower under-detection rates for all Candida species when observed by at least one reader. At 24-h reading, 12 mono-species cultures had correct (or over) detections in all media, whereas 106, 60 and 78 dual-species cultures had correct (or over) detections in BCG, CHROM or SDA, respectively. BCG provides the basis for an accurate laboratory diagnosis of Candida infections.
العلاقة: info:eu-repo/semantics/altIdentifier/pmid/32203515; info:eu-repo/semantics/altIdentifier/wos/WOS:000535303900001; volume:15; issue:3; firstpage:1; lastpage:9; numberofpages:9; issueyear:2020; journal:PLOS ONE; http://hdl.handle.net/10807/166924Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85082102144
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2دورية أكاديمية
المؤلفون: de Carolis E., Pagano L., Murri R., de Pascale G., de Angelis G., Sanguinetti M., Posteraro B.
المساهمون: De Carolis, Elena, Marchionni, F., Torelli, R., Angela, M. G., Pagano, Livio, Murri, Rita, De Pascale, Gennaro, De Angelis, Giulia, Sanguinetti, Maurizio, Posteraro, Brunella
مصطلحات موضوعية: beta-glucan, Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
الوصف: The Fungitell assay (FA) and the Wako β-glucan test (GT) are employed to measure the serum/plasma 1,3-β-D-glucan (BDG), a well-known invasive fungal disease biomarker. Data to convincingly and/or sufficiently support the GT as a valuable alternative to the FA are yet limited. In this study, we evaluated the FA and the GT to diagnose invasive aspergillosis (IA), invasive candidiasis (IC), and Pneumocystis jirovecii pneumonia (PJP). The FA and GT performances were compared in sera of patients with IA (n = 40), IC (n = 78), and PJP (n = 17) with respect to sera of control patients (n = 187). Using the manufacturer's cutoff values of 80 pg/mL and 11 pg/mL, the sensitivity and specificity for IA diagnosis were 92.5% and 99.5% for the FA and 60.0% and 99.5% for the GT, respectively; for IC diagnosis were 100.0% and 97.3% for the FA and 91.0% and 99.5% for the GT, respectively; for PJP diagnosis were 100.0% and 97.3% for the FA and 88.2% and 99.5% for the GT, respectively. When an optimized cutoff value of 7.0 pg/mL for the GT was used, the sensitivity and specificity were 80.0% and 97.3% for IA diagnosis, 98.7% and 97.3% for IC diagnosis, and 94.1% and 97.3% for PJP diagnosis, respectively. At the 7.0-pg/mL GT cutoff, the agreement between the assays remained and/or became excellent for IA (95.1%), IC (97.3%), and PJP (96.5%), respectively. In conclusion, we show that the GT performed as well as the FA only with a lowered cutoff value for positivity. Further studies are expected to establish the equivalence of the two BDG assays.
العلاقة: info:eu-repo/semantics/altIdentifier/pmid/32726358; info:eu-repo/semantics/altIdentifier/wos/WOS:000556908900001; volume:15; issue:7; firstpage:1; lastpage:12; numberofpages:12; issueyear:2020; journal:PLOS ONE; http://hdl.handle.net/10807/166932Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85088850758
