مورد إلكتروني
A role for phosphatidic acid in the formation of “supersized” lipid droplets
| العنوان: | A role for phosphatidic acid in the formation of “supersized” lipid droplets |
|---|---|
| بيانات النشر: | Public Library of Science 2011 |
| تفاصيل مُضافة: | Fei, W Shui, G Zhang, Y Krahmer, N Ferguson, C Kapterian, T Lin, RCY Dawes, IW Brown, AJ Li, P Huang, X Parton, R Wenk, M Walther, TC Yang, H |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Lipid droplets (LDs) are important cellular organelles that govern the storage and turnover of lipids. Little is known about how the size of LDs is controlled, although LDs of diverse sizes have been observed in different tissues and under different (patho)physiological conditions. Recent studies have indicated that the size of LDs may influence adipogenesis, the rate of lipolysis and the oxidation of fatty acids. Here, a genome-wide screen identifies ten yeast mutants producing “supersized” LDs that are up to 50 times the volume of those in wild-type cells. The mutated genes include: FLD1 which encodes a homologue of mammalian seipin; five genes (CDS1, INO2, INO4, CHO2 and OPI3) that are known to regulate phospholipid metabolism; two genes (CKB1 and CKB2) encoding subunits of the casein kinase 2; and two genes (MRPS35 and RTC2) of unknown function. Biochemical and genetic analyses reveal that a common feature of these mutants is an increase in the level of cellular phosphatidic acid (PA). Results from in vivo and in vitro analyses indicate that PA may facilitate the coalescence of contacting LDs, resulting in the formation of “supersized” LDs. In summary, our results provide important insights into how the size of LDs is determined and identify novel gene products that regulate phospholipid metabolism. |
| مصطلحات الفهرس: | seipin, lipin, lipid droplets, phosphatidic acid, 0604 Genetics, text, Journal Article, Article |
| URL: | PLOS Genetics, 7, 7, e1002201 1-e1002201 11 |
| الإتاحة: | Open access content. Open access content https://creativecommons.org/licenses/by-nc-nd/4.0Test free_to_read |
| ملاحظة: | English |
| أرقام أخرى: | LJ1 oai:unsworks.library.unsw.edu.au:1959.4/unsworks_39775 10.1371/journal.pgen.1002201 urn:issn:1553-7404 1031080989 |
| المصدر المساهم: | UNIV OF NEW S WALES From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1031080989 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |