التفاصيل البيبلوغرافية
| العنوان: |
Stimulation of HERG Channel Activity by β-catenin. |
| المؤلفون: |
Munoz, Carlos1, Saxena, Ambrish1, Pakladok, Tatsiana1, Bogatikov, Evgenii1, Wilmes, Jan1, Seebohm, Guiscard2, Föller, Michael1,3, Lang, Florian1 florian.lang@uni-tuebingen.de, Uversky, Vladimir N.4 |
| المصدر: |
PLoS ONE. Aug2012, Vol. 7 Issue 8, Special section p1-5. 5p. |
| مصطلحات موضوعية: |
*GENE expression, *CELL proliferation, *CELL membranes, *XENOPUS eggs, *ACTINOMYCIN, *BREFELDIN |
| مستخلص: |
The multifunctional protein β-catenin governs as transcription factor the expression of a wide variety of genes relevant for cell proliferation and cell survival. In addition, β-catenin is localized at the cell membrane and may influence the function of channels. The present study explored the possibility that beta;-catenin participates in the regulation of the HERG K+ channel. To this end, HERG was expressed in Xenopus oocytes with or without β-catenin and the voltage-gated current determined utilizing the dual electrode voltage clamp. As a result, expression of β-catenin markedly upregulated HERG channel activity, an effect not sensitive to inhibition of transcription with actinomycin D (10 µM). According to chemiluminescence, β-catenin may increase HERG channel abundance within the oocyte cell membrane. Following inhibition of channel insertion into the cell membrane by brefeldin A (5 mM) the decay of current was similar in oocytes expressing HERG together with β-catenin to oocytes expressing HERG alone. The experiments uncover a novel function of APC/β-catenin, i.e. the regulation of HERG channels. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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